The goal of the study was to develop the instruction module of the e-storybooks reading comprehension in the elementary school and test the effect of the instruction module of the e-storybooks reading comprehension. This study was to compare the influences of print-storybooks and the instruction module of the e-storybooks reading comprehension among the fifth graders' reading comprehension ability. The results of the study indicated that there was significant difference between the experimental group and the controlled group. The controlled group had better performances of reading comprehension than experimental group. To put it more specifically, students and teachers were busy learning how to operate the module and platform, and that resulted in greatly reduced learning effect. However, with the mastery of e-storybooks instruction module, experimental group class expressed higher motivation than controlled group class in learning activities. These data indicated that the study had the positive contribution.