Effects of thermosensitive chitosan-gelatin based hydrogel containing glutathione on Cisd2-deficient chondrocytes under oxidative stress

Yung Hsin Cheng, Eddy Chavez, Kun-Ling Tsai, Kai Chiang Yang, Wei Ting Kuo, Yi Ping Yang, Shih Hwa Chiou, Feng Huei Lin

研究成果: Article

5 引文 (Scopus)

摘要

Aging is considered as a primary risk factor in the development of osteoarthritis (OA) which associated with mitochondrial dysfunction and oxidative stress. CDGSH iron sulfur domain 2 (Cisd2) deficiency causes mitochondrial dysfunction and drive premature aging. In the present study, thermosensitive chitosan-gelatin based hydrogel containing glutathione was developed as injectable drug delivery system for administration by minimal invasive surgery for the treatment of OA. Cisd2 deficiency (Cisd2−/−) mouse induced pluripotent stem cells-derived chondrocytes were established and characterized. The results suggested that 100 μM of glutathione may be an optimal concentration to treat Cisd2−/− chondrocytes without cytotoxicity. The developed hydrogel showed sustained release profile of the glutathione and could decrease the reactive oxygen species level. Post-treatment of glutathione-loaded hydrogel could rescue Cisd2−/− chondrocytes from oxidative damage via increasing catalase activity, down-regulation of inflammation, and decreasing apoptosis. These results suggest that thermosensitive glutathione-loaded hydrogel may be a potential antioxidant therapeutic strategy for treating Cisd2−/− chondrocytes in the near future.

原文English
頁(從 - 到)17-27
頁數11
期刊Carbohydrate Polymers
173
DOIs
出版狀態Published - 2017 十月 1

指紋

Oxidative stress
Hydrogel
Chitosan
Gelatin
Sulfur
Hydrogels
Glutathione
Iron
Aging of materials
Cell death
Cytotoxicity
Stem cells
Antioxidants
Catalase
Surgery
Reactive Oxygen Species
Apoptosis
Oxygen

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

引用此文

Cheng, Yung Hsin ; Chavez, Eddy ; Tsai, Kun-Ling ; Yang, Kai Chiang ; Kuo, Wei Ting ; Yang, Yi Ping ; Chiou, Shih Hwa ; Lin, Feng Huei. / Effects of thermosensitive chitosan-gelatin based hydrogel containing glutathione on Cisd2-deficient chondrocytes under oxidative stress. 於: Carbohydrate Polymers. 2017 ; 卷 173. 頁 17-27.
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Effects of thermosensitive chitosan-gelatin based hydrogel containing glutathione on Cisd2-deficient chondrocytes under oxidative stress. / Cheng, Yung Hsin; Chavez, Eddy; Tsai, Kun-Ling; Yang, Kai Chiang; Kuo, Wei Ting; Yang, Yi Ping; Chiou, Shih Hwa; Lin, Feng Huei.

於: Carbohydrate Polymers, 卷 173, 01.10.2017, p. 17-27.

研究成果: Article

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AU - Kuo, Wei Ting

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AU - Chiou, Shih Hwa

AU - Lin, Feng Huei

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