Efficient and stable enzyme immobilization in a block copolypeptide vesicle-templated biomimetic silica support

Jun Kun Lai, Tzu Han Chuang, Jeng Shiung Jan, Steven Sheng Shih Wang

研究成果: Article同行評審

33 引文 斯高帕斯(Scopus)

摘要

We report the immobilization of a model enzyme, papain, within silica matrices by combining vesiclization of poly-l-lysine-b-polyglycine block copolypeptides with following silica mineralization. Our novel strategy utilizes block polypeptide vesicles to induce the condensation of orthosilicic acid while trapping an enzyme within and between vesicles. The polypeptide mediated silica-immobilized enzyme exhibits enhanced pH and thermal stability and reusability, comparing with the free and vesicle encapsulated enzyme. The enhanced enzymatic activity in the immobilized enzyme is due to the confinement of the enzyme in the polypeptide mediated silica matrices. Kinetic analysis shows that the enzyme functionality is determined by the structure and property of silica/polypeptide matrices. The proposed novel strategy provides an alternative route for the synthesis of a broad range of functional bionanocomposites entrapped within silica nanostructures.

原文English
頁(從 - 到)51-58
頁數8
期刊Colloids and Surfaces B: Biointerfaces
80
發行號1
DOIs
出版狀態Published - 2010 十月 1

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 表面和介面
  • 物理與理論化學
  • 膠體和表面化學

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