Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation

Nurul Amirah Hassan, Sugenendran Supramani, Mohamad Nor Azzimi Sohedein, Siti Rokhiyah Ahmad Usuldin, Anita Klaus, Zul Ilham, Wei Hsin Chen, Wan Abd Al Qadr Imad Wan-Mohtar

研究成果: Article同行評審

14 引文 斯高帕斯(Scopus)

摘要

A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Avala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642.8 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (Knuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4–6), temperature (20°C–30 °C), and glucose concentration (10 g/L–50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p < 0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30 °C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.

原文English
文章編號101305
期刊Biocatalysis and Agricultural Biotechnology
21
DOIs
出版狀態Published - 2019 九月

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 生物工程
  • 食品科學
  • 應用微生物與生物技術
  • 農學與作物科學

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