Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation

Nurul Amirah Hassan, Sugenendran Supramani, Mohamad Nor Azzimi Sohedein, Siti Rokhiyah Ahmad Usuldin, Anita Klaus, Zul Ilham, Wei Hsin Chen, Wan Abd Al Qadr Imad Wan-Mohtar

研究成果: Article

2 引文 (Scopus)

摘要

A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Avala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642.8 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (Knuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4–6), temperature (20°C–30 °C), and glucose concentration (10 g/L–50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p < 0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30 °C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.

原文English
文章編號101305
期刊Biocatalysis and Agricultural Biotechnology
21
DOIs
出版狀態Published - 2019 九月

指紋

Reishi
submerged fermentation
Ganoderma lucidum
exopolysaccharides
Mycelium
Biomass
Fermentation
mycelium
Glucose
biomass
glucose
biomass production
Blueprints
Serbia
Temperature
Analysis of variance (ANOVA)
Agaricales
response surface methodology
Analysis of Variance
temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science

引用此文

Hassan, Nurul Amirah ; Supramani, Sugenendran ; Azzimi Sohedein, Mohamad Nor ; Ahmad Usuldin, Siti Rokhiyah ; Klaus, Anita ; Ilham, Zul ; Chen, Wei Hsin ; Wan-Mohtar, Wan Abd Al Qadr Imad. / Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation. 於: Biocatalysis and Agricultural Biotechnology. 2019 ; 卷 21.
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abstract = "A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Avala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642.8 bp) was sequenced and found to be 99{\%} similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (Knuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4–6), temperature (20°C–30 °C), and glucose concentration (10 g/L–50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p < 0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30 °C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.",
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Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation. / Hassan, Nurul Amirah; Supramani, Sugenendran; Azzimi Sohedein, Mohamad Nor; Ahmad Usuldin, Siti Rokhiyah; Klaus, Anita; Ilham, Zul; Chen, Wei Hsin; Wan-Mohtar, Wan Abd Al Qadr Imad.

於: Biocatalysis and Agricultural Biotechnology, 卷 21, 101305, 09.2019.

研究成果: Article

TY - JOUR

T1 - Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation

AU - Hassan, Nurul Amirah

AU - Supramani, Sugenendran

AU - Azzimi Sohedein, Mohamad Nor

AU - Ahmad Usuldin, Siti Rokhiyah

AU - Klaus, Anita

AU - Ilham, Zul

AU - Chen, Wei Hsin

AU - Wan-Mohtar, Wan Abd Al Qadr Imad

PY - 2019/9

Y1 - 2019/9

N2 - A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Avala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642.8 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (Knuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4–6), temperature (20°C–30 °C), and glucose concentration (10 g/L–50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p < 0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30 °C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.

AB - A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Avala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642.8 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (Knuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4–6), temperature (20°C–30 °C), and glucose concentration (10 g/L–50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p < 0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30 °C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.

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