Efficient biotransformation of L-lysine into cadaverine by strengthening pyridoxal 5’-phosphate-dependent proteins in Escherichia coli with cold shock treatment

Chengfeng Xue, Kai Min Hsu, Wan Wen Ting, Shih Fang Huang, Hung Yi Lin, Sheng Feng Li, Jo Shu Chang, I. Son Ng

研究成果: Article同行評審

12 引文 斯高帕斯(Scopus)

摘要

Cadaverine is a five-carbon diamine which serves as an important biochemical for the synthesis of bio-based nylon. It can be produced by the bioconversion of L-lysine with lysine decarboxylase (CadA; EC 4.1.1.18) and relies on cofactor pyridoxal 5′-phosphate (PLP), thus to recycle PLP from the super-salvage pathway by the genes of pdxH, pdxY, and pdxK in Escherichia coli is crucial and urgent. In this study, the optimal PLP production per gram dry cell weight (i.e., 7008 nmol/g-DCW) increased 30-fold in E. coli BL21 by overexpressing pdxY. Cadaverine production reached 34.7 g/L or 41.2 g/L by in vivo CadA co-expression with plasmids of pJY or pPK. The better conversion was obtained in APK strain (co-expressing CadA and pPK) via whole cell biotransformation, resulting in 97 % and 68 % conversion of 0.4 M and 1.2 M L-lysine to 39.6 g/L and 83.2 g/L cadaverine, respectively. Finally, cold shock treatment of whole-cell biocatalyst showed a significant increasing and achieved the highest cadaverine productivity of 121 g/L/h compared to the previous reports.

原文English
文章編號107659
期刊Biochemical Engineering Journal
161
DOIs
出版狀態Published - 2020 九月 15

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 環境工程
  • 生物工程
  • 生物醫學工程

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