Efficient 1H-NMR quantitation and investigation of N-Acetyl-D-glucosamine (GlcNAc) and N,N'-diacetylchitobiose (GlcNAc)2 from chitin

Fu Chien Liu, Chung Ren Su, Tzi Yi Wu, Shyh Gang Su, Huey Lang Yang, John Han You Lin, Tian Shung Wu

研究成果: Article同行評審

10 引文 斯高帕斯(Scopus)

摘要

A quantitative determination method of N-acetyl-D-glucosamine (GlcNAc) and N,N'-diacetylchitobiose (GlcNAc) 2 is proposed using a proton nuclear magnetic resonance experiment. N-acetyl groups of GlcNAc and (GlcNAc) 2 are chosen as target signals, and the deconvolution technique is used to determine the concentration of the corresponding compound. Compared to the HPLC method, 1H-NMR spectroscopy is simple and fast. The method can be used for the analysis of chitin hydrolyzed products with real-time analysis, and for quantifying the content of products using internal standards without calibration curves. This method can be used to quickly evaluate chitinase activity. The temperature dependence of 1H-NMR spectra (VT-NMR) is studied to monitor the chemical shift variation of acetyl peak. The acetyl groups of products are involved in intramolecular H-bonding with the OH group on anomeric sites. The rotation of the acetyl group is closely related to the intramolecular hydrogen bonding pattern, as suggested by the theoretical data (molecular modeling).

原文English
頁(從 - 到)5828-5843
頁數16
期刊International journal of molecular sciences
12
發行號9
DOIs
出版狀態Published - 2011 九月

All Science Journal Classification (ASJC) codes

  • 催化
  • 分子生物學
  • 光譜
  • 電腦科學應用
  • 物理與理論化學
  • 有機化學
  • 無機化學

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