Electrochemical evaluation of avidin-biotin interaction on self-assembled gold electrodes

Shinn Jyh Ding, Bin Wha Chang, Ching Chou Wu, Min Feng Lai, Hsien Chang Chang

研究成果: Article同行評審

58 引文 斯高帕斯(Scopus)

摘要

The avidin-biotin interaction on 11-mercaptoundecanoic acid self-assembled gold electrodes was investigated by means of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The interfacial properties of the modified electrodes were evaluated in the presence of the Fe(CN) 63-/4- couple redox as a probe. A simple equivalent circuit model with a constant phase element was used to interpret the obtained impedance spectra. The results of cyclic voltammetry showed that the voltammetric behavior of the redox probe was influenced by the electrode surface modification. It is evident that the accumulation of treated substances and the binding of biotin to avidin on the electrode surface resulted in the increasing electron-transfer resistance and the decreasing capacitance. The changes in the electron-transfer resistance on the avidin-modified electrodes were more sensitive than that in the capacitance while detecting biotin over the 2-10 μg/mL concentration. The detection amount can be as low as 20 ng/mL based on the electron-transfer resistance that presented the change of 4.3 kΩ without the use of labels. The development of a rapid, facile, and sensitive method for the quantitation of nanogram quantities of biomolecules utilizing EIS may be achieved.

原文English
頁(從 - 到)3660-3666
頁數7
期刊Electrochimica Acta
50
發行號18
DOIs
出版狀態Published - 2005 六月 10

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)
  • Electrochemistry

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