Electrokinetically driven micro flow cytometers with integrated fiber optics for on-line cell/particle detection

Lung Ming Fu, Ruey Jen Yang, Che Hsin Lin, Yu Jen Pan, Gwo Bin Lee

研究成果: Article同行評審

183 引文 斯高帕斯(Scopus)

摘要

This paper presents an innovative micro flow cytometer which is capable of counting and sorting cells or particles. This compact device employs electrokinetic forces rather than the more conventional hydrodynamic forces technique for flow focusing and sample switching, and incorporates buried optical fibers for the on-line detection of cells or particles. This design approach results in a compact microfluidic system and an easier integration process. The proposed cytometer integrates several critical modules, namely electrokinetic-focusing devices, built-in control electrodes, buried optical fibers for on-line detection, and electrokinetic flow switches for bio-particle collection. A linear relationship exists between the focused stream width (d) and the focusing ratio (F/φ), which is estimated to be D≈134.5-53.8F/ φ. The relationship between the particle velocity (U) and the applied voltage (V) is also investigated. Numerical and experimental data confirm the effectiveness of the device when applied to the counting and sorting of 10μm diameter particles and red blood cells.

原文English
頁(從 - 到)163-169
頁數7
期刊Analytica Chimica Acta
507
發行號1
DOIs
出版狀態Published - 2004 四月 1

All Science Journal Classification (ASJC) codes

  • 分析化學
  • 生物化學
  • 環境化學
  • 光譜

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