Electrokinetics-Based Microfluidic Technology for the Rapid Separation and Concentration of Bacteria/Cells/Biomolecules

I. Fang Cheng, Tzu Ying Chen, Hsien Chang Chang

研究成果: Conference contribution

4 引文 斯高帕斯(Scopus)

摘要

Conventional techniques for detection of bacteria/cell and assessment of cancer cell typically use DNA techniques, Western blot and ELISA kits that are high cost, complicated processes and long time consuming. Our researches focus on rapid, portable, simple and highly sensitive separation and detection of cells/bacteria/biomolecules for field-use diagnosis. An ideal portable biosensor (molecular or whole cells detections) unit must have several important features: rapid detection time (<10 minutes), high sensitivity (pM level for molecular detection, 103 cells/ml for whole cell detection), high specificity, small and inexpensive instrumentation configuration. Electrochemical impedance/conductance sensing is preferred over optical detection because of cost and portability concerns. Cancer cell detection using heterogeneous medical samples require continuous isolation, sorting, and trapping of the target bioparticles and immunocolloids within a diagnostic chip. We have developed several electrokinetic strategies to rapid separation, concentration and detection of cells/bacteria/biomolecules in a microfluidic chip using such as dielectrophoresis (DEP), traveling-wave dielectrophoresis (twDEP) and electrohydrodynamics (EHD). Several key techniques we done, which on a rapid/simple/label-free detection platform for the highly sensitive on-chip separation/identification/quantification will be introduced in this paper.

原文English
主出版物標題Key Engineering Materials - Development and Application
發行者Trans Tech Publications
頁面347-351
頁數5
ISBN(列印)9783038350668
DOIs
出版狀態Published - 2014
事件4th International Conference on Key Engineering Materials, ICKEM 2014 - Bali, Indonesia
持續時間: 2014 三月 222014 三月 23

出版系列

名字Advanced Materials Research
911
ISSN(列印)1022-6680

Other

Other4th International Conference on Key Engineering Materials, ICKEM 2014
國家/地區Indonesia
城市Bali
期間14-03-2214-03-23

All Science Journal Classification (ASJC) codes

  • 工程 (全部)

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