Electrooxidation of NADH with Co(phen)33+ by NADH dehydrogenase purified from Bacillus stearothermophilus

Tomokazu Matsue, Hiroshi Yamada, Hsien Chang Chang, Isamu Uchida

研究成果: Article同行評審

24 引文 斯高帕斯(Scopus)

摘要

The NADH dehydrogenase purified from Bacillus stearothermophilus catalyzes effectively the electrochemical oxidation of NADH mediated by Co(phen)3+3. Stopped-flow measurements indicate that the rate constant for the oxidation of the reduced enzyme by Co(phen)3+3 is 5 × 106 M-1 s-1 and that for the oxidation of NADH by the oxidized enzyme > 3 × 107 M-1 s-1. The voltammograms, showing catalytic prepeaks, were simulated using the above rate constants.

原文English
頁(從 - 到)347-354
頁數8
期刊Bioelectrochemistry and Bioenergetics
24
發行號3
DOIs
出版狀態Published - 1990 十二月

All Science Journal Classification (ASJC) codes

  • 生物物理學
  • 物理與理論化學
  • 電化學

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