TY - JOUR
T1 - Electrooxidation of NADH with Co(phen)3+3 by NADH dehydrogenase purified from Bacillus stearothermophilus
AU - Matsue, Tomokazu
AU - Yamada, Hiroshi
AU - Chang, Hsien Chang
AU - Uchida, Isamu
N1 - Funding Information:
The present study was partly supported by Asahi t_~lass i'<~undation and (.h','mts-in-Aid for Scientific Research (Nos. 01470076 and 01~5175) from the Ministry of Education and Culture, Japan.
PY - 1990/12
Y1 - 1990/12
N2 - The NADH dehydrogenase purified from Bacillus stearothermophilus catalyzes effectively the electrochemical oxidation of NADH mediated by Co(phen)3+3. Stopped-flow measurements indicate that the rate constant for the oxidation of the reduced enzyme by Co(phen)3+3 is 5×106 M-1 s-1 and that for the oxidation of NADH by the oxidized enzyme > 3×107 M-1 s-1. The voltammograms, showing catalytic prepeaks, were simulated using the above rate constants.
AB - The NADH dehydrogenase purified from Bacillus stearothermophilus catalyzes effectively the electrochemical oxidation of NADH mediated by Co(phen)3+3. Stopped-flow measurements indicate that the rate constant for the oxidation of the reduced enzyme by Co(phen)3+3 is 5×106 M-1 s-1 and that for the oxidation of NADH by the oxidized enzyme > 3×107 M-1 s-1. The voltammograms, showing catalytic prepeaks, were simulated using the above rate constants.
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U2 - 10.1016/0022-0728(90)87535-R
DO - 10.1016/0022-0728(90)87535-R
M3 - Article
AN - SCOPUS:44949291521
SN - 0022-0728
VL - 299
SP - 347
EP - 354
JO - Journal of Electroanalytical Chemistry
JF - Journal of Electroanalytical Chemistry
IS - 3
ER -