Elevated ALCAM shedding in colorectal cancer correlates with poor patient outcome

Amanda G. Hansen, Tanner J. Freeman, Shanna A. Arnold, Alina Starchenko, Celestial R. Jones-Paris, Michael A. Gilger, Mary K. Washington, Kang Hsien Fan, Yu Shyr, Robert D. Beauchamp, Andries Zijlstra

研究成果: Article同行評審

26 引文 斯高帕斯(Scopus)

摘要

Molecular biomarkers of cancer are needed to assist histologic staging in the selection of treatment, outcome risk stratification, and patient prognosis. This is particularly important for patients with early-stage disease. We show that shedding of the extracellular domain of activated leukocyte cell adhesion molecule (ALCAM) is prognostic for outcome in patients with colorectal cancer (CRC). Previous reports on the prognostic value of ALCAM expression in CRC have been contradictory and inconclusive. This study clarifies the prognostic value of ALCAM by visualizing ectodomain shedding using a dual stain that detects both the extracellular and the intracellular domains in formalin-fixed tissue. Using this novel assay, 105 patients with primary CRCs and 12 normal mucosa samples were evaluated. ALCAM shedding, defined as detection of the intracellular domain in the absence of the corresponding extracellular domain, was significantly elevated in patients with CRC and correlated with reduced survival. Conversely, retention of intact ALCAM was associated with improved survival, thereby confirming that ALCAM shedding is associated with poor patient outcome. Importantly, analysis of patients with stage II CRC showed that disease-specific survival is significantly reduced for patients with elevated ALCAM shedding (P1/40.01; HR, 3.0), suggesting that ALCAM shedding can identify patients with early-stage disease at risk of rapid progression. Cancer Res; 73(10); 2955-64.

原文English
頁(從 - 到)2955-2964
頁數10
期刊Cancer Research
73
發行號10
DOIs
出版狀態Published - 2013 五月 15

All Science Journal Classification (ASJC) codes

  • 腫瘤科
  • 癌症研究

指紋

深入研究「Elevated ALCAM shedding in colorectal cancer correlates with poor patient outcome」主題。共同形成了獨特的指紋。

引用此