Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies

Wen Tso Liu, Jer Horng Wu, Emily Sze Ying Li, Ezrein Shah Selamat

研究成果: Article同行評審

38 引文 斯高帕斯(Scopus)

摘要

The effects of temperature, salt concentration, and formamide concentration on the emission characteristics of commonly used fluorescent labels were evaluated on DNA microchips. The emission intensities of different fluorophores without hybridization were observed to vary, each to a different extent, to mainly temperature changes. Rhodamine red, TAMRA (tetramethylrhodamine), and dyes from the carbocyanide group exhibited the largest variations, and Texas Red and Oregon Green exhibited the smallest variations. This temperature dependency was shown to affect results obtained during melting curve analysis in DNA microarray studies. To minimize the bias associated with the temperature-dependent emission of different fluorescent labels, a normalization step was proposed.

原文English
頁(從 - 到)6453-6457
頁數5
期刊Applied and environmental microbiology
71
發行號10
DOIs
出版狀態Published - 2005 10月

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 食品科學
  • 應用微生物與生物技術
  • 生態學

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