Enhanced carbon capture and utilization (CCU) using heterologous carbonic anhydrase in Chlamydomonas reinhardtii for lutein and lipid production

Jia Yi Lin, Sefli Sri Wahyu Effendi, I. Son Ng

研究成果: Article同行評審

摘要

Chlamydomonas reinhardtii is a model microalga that has a higher growth rate and produces high levels of lutein and lipids, but biomass production is limited. Carbonic anhydrase (CA) converts atmospheric CO2 to bicarbonate which is crucial for carbon‐concentrating mechanism (CCM) in microalgae and boosts cell density. Therefore, C. reinhardtii harboring the heterologous CA from Mesorhizobium loti (MlCA) and Sulfurihydrogenibium yellowstonense (SyCA) were explored to increase CO2 capture and utilization (CCU) through different culture devices. Genetically modified C. reinhardtii was able to grow from mixotrophic to autotrophic conditions. Subsequently, biomass, lutein, and lipid were maximized to OD680 of 4.56, 21.32 mg/L and 672 mg/L using photo-bioreactor (PBR) with 5% CO2. Moreover, CO2 assimilation rate was 2.748 g-CO2/g-DCW and 2.792 g-CO2/g-DCW under mixotrophic and autotrophic conditions, respectively. The biomass accumulation correlated with CA activity. In addition, the transcript levels of major genes in metabolic pathways of lutein and lipid were dramatically increased.

原文English
文章編號127009
期刊Bioresource technology
351
DOIs
出版狀態Published - 2022 5月

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 環境工程
  • 可再生能源、永續發展與環境
  • 廢物管理和處置

指紋

深入研究「Enhanced carbon capture and utilization (CCU) using heterologous carbonic anhydrase in Chlamydomonas reinhardtii for lutein and lipid production」主題。共同形成了獨特的指紋。

引用此