Enhanced live cell membrane imaging using surface plasmon-enhanced total internal reflection fluorescence microscopy

Ruei Yu He, Guan Liang Chang, Hua Lin Wu, Chi Hung Lin, Kuo Chih Chiu, Yuan Deng Su, Shean Jen Chen

研究成果: Article同行評審

43 引文 斯高帕斯(Scopus)

摘要

Using a total internal reflection fluorescence microscopy (TIRFM) technique to image live cells on a biosurface not only provides an enhanced understanding of cellular functions, but also improves the signal-to-noise ratio of the images. However, the intensity of the fluorescence signal must be increased if a more dynamic biomolecular imaging capability is required. Accordingly, this study presents a surface plasmon-enhanced TIRFM technique in which the fluorescence signals are enhanced via surface plasmons offered by a silver nanolayer. The developed microscopy technique is successfully applied to the real-time observation of the thrombomodulin proteins of live cell membranes. The experimental results and the simulation results demonstrate that the live cell membrane images obtained in the proposed surface plasmon-enhanced TIRFM technique are brighter by approximately one order of magnitude than those provided by conventional TIRFM.

原文English
頁(從 - 到)9307-9316
頁數10
期刊Optics Express
14
發行號20
DOIs
出版狀態Published - 2006 十月

All Science Journal Classification (ASJC) codes

  • 原子與分子物理與光學

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