Enhanced mobile hybridization of gold nanoparticles decorated with oligonucleotide in microchannel devices

Miao Hsing Hsu, Wei Feng Fang, Yu Hsuan Lai, Jing Tang Yang, Tsung Lin Tsai, Dar Bin Shieh

研究成果: Conference contribution

摘要

We have designed a microfluidic device that is capable of enhancing passive mixing through the micro-structures, positioned along the channels of the device. We investigated the capability of these structures to promote mobile hybridization of fluorophore-labeled target oligonucleotides to Au-NP probes. This process is monitored with fluorescence through the quenching of the fluorescent signal within the device as the target oligonucleotides become bound to the Au-NP probes. We evaluated the fluorescent intensity of a sample image which was completed in about 7.2 s. Mobile hybridization is thus much more effective than traditional static hybridization limited by molecular diffusion.

原文English
主出版物標題14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
頁面336-338
頁數3
出版狀態Published - 2010
事件14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
持續時間: 2010 10月 32010 10月 7

出版系列

名字14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
1

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
國家/地區Netherlands
城市Groningen
期間10-10-0310-10-07

All Science Journal Classification (ASJC) codes

  • 控制與系統工程

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