Enhanced two-photon excited fluorescence in three-dimensionally crosslinked bovine serum albumin microstructures

Keng Chi Cho, Chi Hsiang Lien, Chun Yu Lin, Chia Yuan Chang, Lynn L.H. Huang, Paul J. Campagnola, Chen Yuan Dong, Shean Jen Chen

研究成果: Article同行評審

17 引文 斯高帕斯(Scopus)

摘要

In this study, the intensity of two-photon excited fluorescence (TPEF) of xanthene dye, Rose Bengal (RB), was significantly enhanced via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking (TPC) technique. The RB was utilized as the photoactivator in the TPC processing and the enhanced TPEF intensity correlates with the concentration of fabricated crosslinked BSA microstructures via the power control and pulse selection of the employed femtosecond laser. As a result, fabrication of three-dimensional BSA microstructures can be simultaneously monitored by the use of TPEF intensity. The crosslinked BSA microstructures synthesized may be used as an ordered biomaterial for fluorescence enhancement.

原文English
頁(從 - 到)11732-11739
頁數8
期刊Optics Express
19
發行號12
DOIs
出版狀態Published - 2011 6月 6

All Science Journal Classification (ASJC) codes

  • 原子與分子物理與光學

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