Enhanced viability of a nervous necrosis virus-infected stable cell line over-expressing a fusion product of the zfBcl-x L and green fluorescent protein genes

S. P. Chen, H. L. Yang, H. Y. Lin, M. C. Chen, J. L. Wu, Jiann-Ruey Hong

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

Nervous necrosis virus (NNV) infection induces host cell apoptosis by an ill-understood process. We utilized a fusion between enhanced green fluorescent protein (EGFP) and the zfBcl-xL gene in GL-av cells to select for zfBcl-xL stable cell lines and to assess the effectiveness of the anti-apoptotic protein Bcl-xL in circumventing NNV-induced cell death. Stable EGFP and EGFP-Bcl-xL-expressing clones were obtained at high purity within 2.5-3 months. In the latter, the EGFP-Bcl-xL fusion protein (approximately 58.2 kDa, as ascertained by Western blot) was predominantly targeted to mitochondria. We assayed for apoptosis in red-spotted grouper NNV Tainan no. 1 (RGNNV TN1)-infected cells with terminal deoxynucleotidyl transferase (TdT)-mediated end labelling (TUNEL) of DNA at different virus doses. NNV infection of NNV Bcl-xL GL-av cell line revealed a protective effect, with a decrease in TUNEL-positive cells of 7%, 8% and 31.8% at 24, 48 and 72 h, respectively. In addition, RGNNV infection of the Bcl-xL GL-av cell line revealed a protective effect, with an enhanced viability of 3%, 40% and 73% at 24, 48, and 72 h, respectively. We conclude that NNV-induced apoptotic cell death can be lessened in transgenic grouper fish cells.

原文English
頁(從 - 到)347-354
頁數8
期刊Journal of Fish Diseases
29
發行號6
DOIs
出版狀態Published - 2006 六月 1

All Science Journal Classification (ASJC) codes

  • 海洋科學
  • 獸醫(雜項)

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