Enhancement of an electroporation system for gene delivery using electrophoresis with a planar electrode

Keng Shiang Huang, Yu Cheng Lin, Chi Chang Su, Chun Sheng Fang

研究成果: Article同行評審

27 引文 斯高帕斯(Scopus)

摘要

In this paper a new electroporation (EP) system is developed, which includes an EP microchip and a logic circuit, which combined with electrophoresis (ES), can provide site-specific enhancement of gene concentration. In this ES-EP microchip, an arc planar electrode provides the ES function for DNA attraction, and interdigitated array electrodes provide appropriate electric fields for the EP on the chip surface. In addition, the adherent cells can be manipulated in situ without detachment of the ES-EP microchip, which performs the "Lab on a chip". Experimental results have shown that the efficiency of gene transfection with an attracting-electric field (35.89%) becomes much higher than that without an attracting-electric field (16.62%). Cell numbers as low as 104 cells, and DNA as little as 4 μg are sufficient for evaluating the phenotypic effects following the over-expression of the introduced genes on the ES-EP microchip. The proposed system has the advantages of portability, cost-effectiveness, a high transfection rate and ease of operation.

原文English
頁(從 - 到)86-92
頁數7
期刊Lab on a Chip
7
發行號1
DOIs
出版狀態Published - 2007 一月 1

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 生物化學
  • 化學 (全部)
  • 生物醫學工程

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