Enhancement of an electroporation system for gene delivery using electrophoresis with planar electrodes

Keng Shiang Huang, Min Li, Chi Chang Su, Hau Hsuan Huang, Chun Sheng Fang, Yu Cheng Lin

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

We developed a new electroporation system, including a microchip and a logic circuit, and combining with the function of electrophoresis, which can site-specific enhancement of the gene concentration. We have demonstrated that the electroporation microchip could enhance and target in vitro gene transfection for cell lines. In this micro-device, the outer electrodes could provide the electrophoresis function for DNA attraction, and the inner electrodes could provide appropriate electric fields for the electroporation on the chip surface. The electrostatic force can be designed into specific regions, where the DNA plasmids are attracted to provide the region-targeting function. This study successfully demonstrates that the electrostatic force can attract DNA plasmids to the cell surface and highly enhance the gene delivery. Experimental results showed that the efficiency of gene transfection with an attracting-electric field become much higher than that without an attracting-electric field. Furthermore, the adherent cells could be manipulated in situ without detachment by this EP microchip. The system has several advantages of portable, cost-effective, high transfection rate and easy operation.

原文English
主出版物標題Proceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
頁面522-525
頁數4
出版狀態Published - 2005 十二月 1
事件2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005 - Shanghai, China
持續時間: 2005 九月 12005 九月 4

出版系列

名字Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
7 VOLS
ISSN(列印)0589-1019

Other

Other2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
國家/地區China
城市Shanghai
期間05-09-0105-09-04

All Science Journal Classification (ASJC) codes

  • 訊號處理
  • 生物醫學工程
  • 電腦視覺和模式識別
  • 健康資訊學

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