Enhancement of antitumor immune response by targeted interleukin-12 electrogene transfer through antiHER2 single-chain antibody in a murine bladder tumor model

Yuh Shyan Tsai, Ai Li Shiau, Yu Fon Chen, Hsin Tzu Tsai, Hwei Ling Lee, Tzong Shin Tzai, Chao Liang Wu

研究成果: Article同行評審

12 引文 斯高帕斯(Scopus)

摘要

Interleukin-12 (IL-12), despite exerting antitumor activity, has limited therapeutic uses due to its systemic toxicity. Since HER2 (also known as ErbB-2, neu, and HER2/neu) is frequently overexpressed on cancer cells, HER2-targeted delivery of IL-12 to tumors may be a promising strategy for enhancing antitumor immunity. Here we showed that intramuscular electrogene transfer of an expression vector encoding a fusion protein antiHER2scFv-IL12, which consists of antiHER2 single-chain variable fragment (scFv) and single-chain IL-12, significantly retarded tumor growth and prolonged the survival in a syngeneic bladder tumor model. Elevated IL-12 and interferon-γ (IFN-γ) levels, increased infiltration of CD4+ and CD8+ T cells, and reduced vascular endothelial growth factor (VEGF) expression in the tumors, as well as enhanced cytolytic activity of splenocytes were noted in the treated mice. Our results suggest that this approach may be effective for the treatment of HER2-overexpressing tumors.

原文English
頁(從 - 到)5383-5392
頁數10
期刊Vaccine
27
發行號39
DOIs
出版狀態Published - 2009 八月 27

All Science Journal Classification (ASJC) codes

  • 分子醫學
  • 免疫學與微生物學 (全部)
  • 獸醫 (全部)
  • 公共衛生、環境和職業健康
  • 傳染性疾病

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