Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane

I. Son Ng, Malcolm S.Y. Tang, Pau Loke Show, Zih Ming Chiou, Jung Chin Tsai, Yu Kaung Chang

研究成果: Article

3 引文 斯高帕斯(Scopus)

摘要

In this work, a chitosan-modified nanofiber membrane was fabricated and used to examine the permeation characteristics of C-phycocyanin (CPC) obtained from Spirulina platensis. The effects of NaCl concentration (0.1–1.0 M), chitosan coupling pH (6–8), chitosan coupling concentration (0.1–3.0%), algal solution pH (6–8), algal mass concentration (0.1–1.0% dw/v), and membrane flux (4.08 × 10 −2 –2.04 × 10 −1 mL/min·cm 2 ) on the penetration performance of the membrane for CPC were investigated. The results show that the order of binding selectivity of the membrane for these proteins is contaminating proteins (TP) > allophycocyanin (APC) > CPC. TP and APC molecules were more easily adsorbed by the chitosan-modified membrane, and the CPC molecules most easily penetrated the membrane without being adsorbed, enhancing CPC purity. The purification factor and total mass flux were 3.3 fold and 66%, respectively, in a single step.

原文English
頁(從 - 到)615-628
頁數14
期刊International Journal of Biological Macromolecules
132
DOIs
出版狀態Published - 2019 七月 1

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • Energy(all)

指紋 深入研究「Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane」主題。共同形成了獨特的指紋。

  • 引用此