TY - JOUR
T1 - Enhancement of NMDA receptor-mediated synaptic potential by isoproterenol is blocked by Rp-adenosine 3′,5′-cyclic monophosphothioate
AU - Huang, Chiung Chun
AU - Tsai, Jing Jane
AU - Gean, Po Wu
N1 - Funding Information:
This study was supported by the National Science Council of Taiwan, Republic of China NSC-82-0420-B006-012-M 10).
PY - 1993/10/29
Y1 - 1993/10/29
N2 - The intracellular mechanisms underlying the facilitatory action of isoproterenol (Iso) on the NMDA receptor-mediated synaptic potential (EPSPnmda) was investigated in an in vitro slice preparation of rat amygdala. Intracellular recordings were made from basolateral amygdala neurons in the presence of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 μM) and picrotoxin (50 μM) which block non-NMDA and GABAa receptors, respectively. Superfusion of Iso (15 μM) produced a sustained increase in EPSPnmda. Rp-adenosine-3′,5′-cyclic monophosphothioate (Rp-cAMPS), a potent inhibitor of protein kinase A (PKA) alone decreased the amplitude of EPSPnmda below baseline values and prevented the subsequent potentiation by Iso. Forskolin, a direct activator of adenylate cyclase, mimics the effect of Iso, and Rp-cAMPS also reversed forskolin-induced enhancement of EPSPnmda. These results suggest that cAMP-dependent protein kinase mediates the enhancement of EPSPnmda by Iso in the amygdala.
AB - The intracellular mechanisms underlying the facilitatory action of isoproterenol (Iso) on the NMDA receptor-mediated synaptic potential (EPSPnmda) was investigated in an in vitro slice preparation of rat amygdala. Intracellular recordings were made from basolateral amygdala neurons in the presence of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 μM) and picrotoxin (50 μM) which block non-NMDA and GABAa receptors, respectively. Superfusion of Iso (15 μM) produced a sustained increase in EPSPnmda. Rp-adenosine-3′,5′-cyclic monophosphothioate (Rp-cAMPS), a potent inhibitor of protein kinase A (PKA) alone decreased the amplitude of EPSPnmda below baseline values and prevented the subsequent potentiation by Iso. Forskolin, a direct activator of adenylate cyclase, mimics the effect of Iso, and Rp-cAMPS also reversed forskolin-induced enhancement of EPSPnmda. These results suggest that cAMP-dependent protein kinase mediates the enhancement of EPSPnmda by Iso in the amygdala.
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U2 - 10.1016/0304-3940(93)90295-V
DO - 10.1016/0304-3940(93)90295-V
M3 - Article
C2 - 7903801
AN - SCOPUS:0027361927
SN - 0304-3940
VL - 161
SP - 207
EP - 210
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2
ER -