Enhancement of renal epithelial cell functions through microfluidic-based coculture with adipose-derived stem cells

Hui Chun Huang, Ya Ju Chang, Wan Chun Chen, Hans I.Chen Harn, Ming Jer Tang, Chia Ching Wu

研究成果: Article同行評審

39 引文 斯高帕斯(Scopus)

摘要

Current hemodialysis has functional limitations and is insufficient for renal transplantation. The bioartificial tubule device has been developed to contribute to metabolic functions by implanting renal epithelial cells into hollow tubes and showed a higher survival rate in acute kidney injury patients. In healthy kidney, epithelial cells are surrounded by various types of cells that interact with extracellular matrices, which are primarily composed of laminin and collagen. The current study developed a microfluidic coculture platform to enhance epithelial cell function in bioartificial microenvironments with multiple microfluidic channels that are microfabricated by polydimethylsiloxane. Collagen gel (CG) encapsulated with adipose-derived stem cells (CG-ASC) was injected into a central microfluidic channel for three-dimensional (3D) culture. The resuspended Madin-Darby canine kidney (MDCK) cells were injected into nascent channels and formed an epithelial monolayer. In comparison to coculture different cells using the commercial transwell system, the current coculture device allowed living cell monitoring of both the MDCK epithelial monolayer and CG-ASC in a 3D microenvironment. By coculture with CG-ASC, the cell height was increased with columnar shapes in MDCK. Promotion of cilia formation and functional expression of the ion transport protein in MDCK were also observed in the cocultured microfluidic device. When applying fluid flow, the intracellular protein dynamics can be monitored in the current platform by using the time-lapse confocal microscopy and transfection of GFP-tubulin plasmid in MDCK. Thus, this microfluidic coculture device provides the renal epithelial cells with both morphological and functional improvements that may avail to develop bioartificial renal chips.

原文English
頁(從 - 到)2024-2034
頁數11
期刊Tissue Engineering - Part A
19
發行號17-18
DOIs
出版狀態Published - 2013 9月 1

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 生物化學
  • 生物材料
  • 生物醫學工程

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