TY - JOUR
T1 - Enhancing lutein production with Chlorella sorokiniana Mb-1 by optimizing acetate and nitrate concentrations under mixotrophic growth
AU - Chen, Chun Yen
AU - Ho, Shih Hsin
AU - Liu, Chen Chun
AU - Chang, Jo Shu
N1 - Publisher Copyright:
© 2017
PY - 2017/10
Y1 - 2017/10
N2 - Lutein is a xanthophyll pigment known for its antioxidant activities and its protective role against eye damage. In this study, lutein production from microalgae with mixotrophic cultivation is demonstrated. Four acetate-utilizing microalgae with high lutein accumulating ability were isolated and tested for lutein production. Chlorella sorokiniana Mb-1, with the highest lutein productivity, was chosen for further studies. The effects of acetate and nitrate concentrations in the BG11-based medium were examined. The optimum concentrations of nitrate and acetate were determined using a statistical experimental design methodology. The results show that the optimal composition of acetate and nitrate was 4.88 g/L and 1.83 g/L, respectively, leading to a predicted lutein productivity of 3.96 mg/L/day. This predicted value was verified by experiments indicating that lutein productivity could be enhanced from 2.79 mg/L/day to 3.97 mg/L/day (42% enhancement) via optimizing the carbon and nitrogen sources in the mixotrophic culture medium.
AB - Lutein is a xanthophyll pigment known for its antioxidant activities and its protective role against eye damage. In this study, lutein production from microalgae with mixotrophic cultivation is demonstrated. Four acetate-utilizing microalgae with high lutein accumulating ability were isolated and tested for lutein production. Chlorella sorokiniana Mb-1, with the highest lutein productivity, was chosen for further studies. The effects of acetate and nitrate concentrations in the BG11-based medium were examined. The optimum concentrations of nitrate and acetate were determined using a statistical experimental design methodology. The results show that the optimal composition of acetate and nitrate was 4.88 g/L and 1.83 g/L, respectively, leading to a predicted lutein productivity of 3.96 mg/L/day. This predicted value was verified by experiments indicating that lutein productivity could be enhanced from 2.79 mg/L/day to 3.97 mg/L/day (42% enhancement) via optimizing the carbon and nitrogen sources in the mixotrophic culture medium.
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U2 - 10.1016/j.jtice.2017.04.020
DO - 10.1016/j.jtice.2017.04.020
M3 - Article
AN - SCOPUS:85023647037
SN - 1876-1070
VL - 79
SP - 88
EP - 96
JO - Journal of the Taiwan Institute of Chemical Engineers
JF - Journal of the Taiwan Institute of Chemical Engineers
ER -