TY - JOUR
T1 - Enhancing lutein production with mixotrophic cultivation of Chlorella sorokiniana MB-1-M12 using different bioprocess operation strategies
AU - Chen, Jih Heng
AU - Chen, Chun Yen
AU - Hasunuma, Tomohisa
AU - Kondo, Akihiko
AU - Chang, Chien Hsiang
AU - Ng, I. Son
AU - Chang, Jo Shu
N1 - Funding Information:
The authors gratefully acknowledge financial support received from Taiwan’s Ministry of Science and Technology under grant number 107-3113-E-006-009 , 107-2221-E-006-112-MY3 , 107-2621-M-006-003 , and 107-2218-E-006-016 . The authors also appreciate the financial support of DDS Institute of Advanced Education , Taiwan.
Funding Information:
The authors gratefully acknowledge financial support received from Taiwan's Ministry of Science and Technology under grant number 107-3113-E-006-009, 107-2221-E-006-112-MY3, 107-2621-M-006-003, and 107-2218-E-006-016. The authors also appreciate the financial support of DDS Institute of Advanced Education, Taiwan.
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/4
Y1 - 2019/4
N2 - A lutein-enriched mutant, Chlorella sorokiniana MB-1-M12 was grown mixotrophically for lutein production. The lutein production efficiency of the strain was enhanced via optimizing the operating strategies. The results show that using semi-continuous cultivation with a medium replacement ratio of 75% resulted in a higher lutein productivity and lutein concentration of 6.24 mg/L/d and 50.6 mg/L, respectively, which were markedly higher than those obtained from batch and fed-batch cultivation. Cultivation under simulated outdoor cultivation conditions (i.e., temperature of 35 °C/25 °C for a 12 h/12 h light/dark cycle) could achieve the highest lutein productivity and lutein concentration of 3.34 mg/L/d and 30.8 mg/L, respectively. Lutein production via outdoor cultivation of MB-1-M12 strain with a 60-L tubular photobioreactor was performed using semi-continuous operation. With a medium replacement ratio of 75%, a good lutein productivity (4.46 mg/L/d) and concentration (27.4 mg/L) was obtained, indicating the feasibility of producing lutein under outdoor cultivation of the microalgal strain.
AB - A lutein-enriched mutant, Chlorella sorokiniana MB-1-M12 was grown mixotrophically for lutein production. The lutein production efficiency of the strain was enhanced via optimizing the operating strategies. The results show that using semi-continuous cultivation with a medium replacement ratio of 75% resulted in a higher lutein productivity and lutein concentration of 6.24 mg/L/d and 50.6 mg/L, respectively, which were markedly higher than those obtained from batch and fed-batch cultivation. Cultivation under simulated outdoor cultivation conditions (i.e., temperature of 35 °C/25 °C for a 12 h/12 h light/dark cycle) could achieve the highest lutein productivity and lutein concentration of 3.34 mg/L/d and 30.8 mg/L, respectively. Lutein production via outdoor cultivation of MB-1-M12 strain with a 60-L tubular photobioreactor was performed using semi-continuous operation. With a medium replacement ratio of 75%, a good lutein productivity (4.46 mg/L/d) and concentration (27.4 mg/L) was obtained, indicating the feasibility of producing lutein under outdoor cultivation of the microalgal strain.
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U2 - 10.1016/j.biortech.2019.01.041
DO - 10.1016/j.biortech.2019.01.041
M3 - Article
C2 - 30669027
AN - SCOPUS:85060111773
SN - 0960-8524
VL - 278
SP - 17
EP - 25
JO - Bioresource technology
JF - Bioresource technology
ER -
