Enterovirus 71 virion-associated galectin-1 facilitates viral replication and stability

Pei Huan Lee, Chia Ming Liu, Tzong-Shiann Ho, Yi Che Tsai, Chi Cheng Lin, Ya Fang Wang, Yuh-Ling Chen, Chun-Keung Yu, Shih-Min Wang, Ching-Chuan Liu, Ai-Li Shiau, Huan Yao Lei, Chih-Peng Chang

研究成果: Article

14 引文 (Scopus)

摘要

Enterovirus 71 (EV71) infection causes a myriad of diseases from mild hand-foot-and-mouth disease or herpangina to fatal brain stem encephalitis complicated with pulmonary edema. Several severe EV71 endemics have occurred in Asia-Pacific region, including Taiwan, and have become a serious threat to children' s health. EV71 infection is initiated by the attachment of the virion to the target cell surface. Although this process relies primarily upon interaction between viruses and cell surface receptors, soluble factors may also influence the binding of EV71 to host cells.Galectin-1 has been reported to participate in several virus infections, but is not addressed in EV71. In this study, we found that the serum levels of galectin-1 in EV71-infected children were higher than those in non-infected people. In EV71 infected cells, galectin-1 was found to be associated with the EV71 VP1 and VP3 via carbohydrate residues and subsequently released and bound to another cell surface along with the virus. EV71 propagated from galectin-1 knockdown SK-N-SH cells exhibited lower infectivity in cultured cells and less pathogenicity in mice than the virus propagated from parental cells. In addition, this galectin-1-free EV71 virus was sensitive to high temperature and lost its viability after long-term storage, which could be restored following supplement of recombinant galectin-1. Taken together, our findings uncover a new role of galectin-1 in facilitating EV71 virus infection.

原文English
文章編號e0116278
期刊PloS one
10
發行號2
DOIs
出版狀態Published - 2015 二月 23

指紋

Galectin 1
Enterovirus
virus replication
virion
Virion
Viruses
viruses
Enterovirus Infections
Virus Diseases
cells
Herpangina
infection
Hand, Foot and Mouth Disease
Cell Surface Receptors
pathogenicity
hand, foot and mouth disease
Brain
Pulmonary Edema
Encephalitis
Taiwan

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

引用此文

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title = "Enterovirus 71 virion-associated galectin-1 facilitates viral replication and stability",
abstract = "Enterovirus 71 (EV71) infection causes a myriad of diseases from mild hand-foot-and-mouth disease or herpangina to fatal brain stem encephalitis complicated with pulmonary edema. Several severe EV71 endemics have occurred in Asia-Pacific region, including Taiwan, and have become a serious threat to children' s health. EV71 infection is initiated by the attachment of the virion to the target cell surface. Although this process relies primarily upon interaction between viruses and cell surface receptors, soluble factors may also influence the binding of EV71 to host cells.Galectin-1 has been reported to participate in several virus infections, but is not addressed in EV71. In this study, we found that the serum levels of galectin-1 in EV71-infected children were higher than those in non-infected people. In EV71 infected cells, galectin-1 was found to be associated with the EV71 VP1 and VP3 via carbohydrate residues and subsequently released and bound to another cell surface along with the virus. EV71 propagated from galectin-1 knockdown SK-N-SH cells exhibited lower infectivity in cultured cells and less pathogenicity in mice than the virus propagated from parental cells. In addition, this galectin-1-free EV71 virus was sensitive to high temperature and lost its viability after long-term storage, which could be restored following supplement of recombinant galectin-1. Taken together, our findings uncover a new role of galectin-1 in facilitating EV71 virus infection.",
author = "Lee, {Pei Huan} and Liu, {Chia Ming} and Tzong-Shiann Ho and Tsai, {Yi Che} and Lin, {Chi Cheng} and Wang, {Ya Fang} and Yuh-Ling Chen and Chun-Keung Yu and Shih-Min Wang and Ching-Chuan Liu and Ai-Li Shiau and Lei, {Huan Yao} and Chih-Peng Chang",
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Enterovirus 71 virion-associated galectin-1 facilitates viral replication and stability. / Lee, Pei Huan; Liu, Chia Ming; Ho, Tzong-Shiann; Tsai, Yi Che; Lin, Chi Cheng; Wang, Ya Fang; Chen, Yuh-Ling; Yu, Chun-Keung; Wang, Shih-Min; Liu, Ching-Chuan; Shiau, Ai-Li; Lei, Huan Yao; Chang, Chih-Peng.

於: PloS one, 卷 10, 編號 2, e0116278, 23.02.2015.

研究成果: Article

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AU - Liu, Chia Ming

AU - Ho, Tzong-Shiann

AU - Tsai, Yi Che

AU - Lin, Chi Cheng

AU - Wang, Ya Fang

AU - Chen, Yuh-Ling

AU - Yu, Chun-Keung

AU - Wang, Shih-Min

AU - Liu, Ching-Chuan

AU - Shiau, Ai-Li

AU - Lei, Huan Yao

AU - Chang, Chih-Peng

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