Entrapment and measurement of a biologically functionalized microbead with a microwell electrode

Ching Yu Chang, Yasufumi Takahashi, Tatsuya Murata, Hitoshi Shiku, Hsien Chang Chang, Tomokazu Matsue

研究成果: Article同行評審

20 引文 斯高帕斯(Scopus)

摘要

A chip with integrated electrophoretic and electrochemical systems was developed to manipulate either an individual microbead or a cell inside a microwell electrode (MWE) for electrochemical measurement. The optimal MWE geometry (30 μm diameter and 25 μm depth) was designed to accommodate the micro particles according to the simulated results. A chip device was sequentially built from a slide patterned with Pt electrodes, an adhesive tape defined with a flow channel (200 μm in width and 25 μm in height), and an indium tin oxide (ITO) cover. The MWE not only generated an active electrophoretic force to trap the particle but also provided a low flow velocity area (LFVA) to stabilize the trapped bead or cell in a continuous flow. Scanning electrochemical microscopy (SECM) theory was employed to explain the electrochemical behaviors of the MWE. An enhanced current was confirmed as the redox recycling effect on the conductive ITO cover. The catalytic reaction of an individual alkaline phosphatase coated microbead (ALP-bead) was electrochemically detected with the MWE after being trapped. The ALP on the trapped ALP-bead catalyzed the hydrolysis of p-aminophenylphosphate (PAPP) to p-aminophenol (PAP), and then a decaying amperogram (+0.3 V vs. Ag/AgCl) due to a tiny PAP quantity around the MWE was observed.

原文English
頁(從 - 到)1185-1192
頁數8
期刊Lab on a Chip
9
發行號9
DOIs
出版狀態Published - 2009

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 生物化學
  • 化學 (全部)
  • 生物醫學工程

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