Enzymatic transesterification of microalgal oil from Chlorella vulgaris ESP-31 for biodiesel synthesis using immobilized Burkholderia lipase

Dang Thuan Tran, Kuei Ling Yeh, Ching Lung Chen, Jo Shu Chang

研究成果: Article同行評審

145 引文 斯高帕斯(Scopus)

摘要

An indigenous microalga Chlorella vulgaris ESP-31 grown in an outdoor tubular photobioreactor with CO 2 aeration obtained a high oil content of up to 63.2%. The microalgal oil was then converted to biodiesel by enzymatic transesterification using an immobilized lipase originating from Burkholderia sp. C20. The conversion of the microalgae oil to biodiesel was conducted by transesterification of the extracted microalgal oil (M-I) and by transesterification directly using disrupted microalgal biomass (M-II). The results show that M-II achieved higher biodiesel conversion (97.3wt% oil) than M-I (72.1wt% oil). The immobilized lipase worked well when using wet microalgal biomass (up to 71% water content) as the oil substrate. The immobilized lipase also tolerated a high methanol to oil molar ratio (>67.93) when using the M-II approach, and can be repeatedly used for six cycles (or 288h) without significant loss of its original activity.

原文English
頁(從 - 到)119-127
頁數9
期刊Bioresource technology
108
DOIs
出版狀態Published - 2012 三月

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 環境工程
  • 可再生能源、永續發展與環境
  • 廢物管理和處置

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