Enzyme digestion-based microfluidic system for DNA methylation assay

Chih Hung Wang, Keng-Fu Hsu, Cheng-Yang Chou, Gwo Bin Lee

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

Early and accurate diagnosis of cancer plays a very important role in cancer treatment. The DNA methylation of tumor suppressor genes has been used as a diagnostic biomarker of early carcinogenesis. The 5-methylcytosine of CpG islands in the promoter region has been demonstrated as an evidence of DNA methylation. In this study, the entire process for performing DNA methylation assay including capturing of target DNA, HpaII and MspI endonuclease digestion and nucleic acid amplification have been realized in an integrated microfluidic system. The limit of detection in the microfluidic system was experimentally found to be 10 2 cells/reaction. The entire process from sample loading to results observed only takes 3 hrs. Rapid diagnosis of ovarian cancer cells in the integrated microfluidic system has been demonstrated by using cell lines and clinical samples. The developed micro system may be promising for early diagnosis of cancers.

原文English
主出版物標題2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
頁面985-988
頁數4
DOIs
出版狀態Published - 2012 五月 7
事件2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
持續時間: 2012 一月 292012 二月 2

出版系列

名字Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN(列印)1084-6999

Other

Other2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
國家France
城市Paris
期間12-01-2912-02-02

    指紋

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

引用此

Wang, C. H., Hsu, K-F., Chou, C-Y., & Lee, G. B. (2012). Enzyme digestion-based microfluidic system for DNA methylation assay. 於 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 (頁 985-988). [6170351] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2012.6170351