Enzymolysis kinetics of corn straw by impeded Michaelis model and Box-Behnken design

Peng Liu, Ying Zhao, Hongliang Guo, Jo Shu Chang, Duu Jong Lee

研究成果: Article同行評審

摘要

Enzymatic hydrolysis is an essential step in the lignocellulosic biorefining process. In this paper, Box-Behnken was used to optimize the enzymatic hydrolysis process of corn stalk, and the promotion effect of three typical surfactants on the enzymatic hydrolysis process was investigated. The experimental results showed that the total reducing sugar yield reached 67.6% under the best-predicted conditions. When the concentration of Tween 80 is 0.1%, it could be increased to 80.2%. In addition, the Impeded Michaels Model (IMM) is introduced in this study to describe the enzymatic hydrolysis process of corn stalks. Finally, the initial contact coefficient between the enzyme and cellulose (Kobs,0) and the gradual loss coefficient of enzyme activity (ki) caused by reaction obstruction were obtained by fitting data, which successfully verified the rationality of the model.

原文English
文章編號117658
期刊Environmental Research
242
DOIs
出版狀態Published - 2024 2月 1

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 一般環境科學

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