(−)-Epigallocatechin-3-gallate decreases osteoclastogenesis via modulation of RANKL and osteoprotegrin

Shih Tse Chen, Lin Kang, Chau Zen Wang, Peng Ju Huang, Hsuan Ti Huang, Sung Yen Lin, Shih Hsiang Chou, Cheng Chang Lu, Po Chih Shen, Yi Shan Lin, Chung Hwan Chen

研究成果: Article

9 引文 斯高帕斯(Scopus)

摘要

Osteoporosis is the second most common epidemiologic disease in the aging population worldwide. Previous studies have found that frequent tea drinkers have higher bone mineral density and less hip fracture. We previously found that (−)-epigallocatechin gallate (EGCG) (20–100 µmol/L) significantly suppressed receptor activator of nuclear factor-kB ligand (RANKL)-induced osteoclastogenesis and pit formation via inhibiting NF-κB transcriptional activity and nuclear transport of NF-κB in RAW 264.7 cells and murine primary bone marrow macrophage cells. The most important regulation in osteoclastogenesis is the receptor activator of nuclear factor-kB/ RANKL/osteoprotegrin (RANK/RANKL/OPG) pathway. In this study, we used the coculture of RAW 264.7 cells and the feeder cells, ST2, to evaluate how EGCG regulated the RANK/RANKL/OPG pathway in RAW 264.7 cells and ST2 cells. We found EGCG decreased the RANKL/OPG ratio in both mRNA expression and secretory protein levels and eventually decreased osteoclastogenesis by TRAP (+) stain osteoclasts and TRAP activity at low concentrations—1 and 10 µmol/L—via the RANK/RANKL/OPG pathway. The effective concentration can be easily achieved in daily tea consumption. Taken together, our results implicate that EGCG could be an important nutrient in modulating bone resorption.

原文English
文章編號156
期刊Molecules
24
發行號1
DOIs
出版狀態Published - 2019 一月 3

    指紋

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

引用此

Chen, S. T., Kang, L., Wang, C. Z., Huang, P. J., Huang, H. T., Lin, S. Y., Chou, S. H., Lu, C. C., Shen, P. C., Lin, Y. S., & Chen, C. H. (2019). (−)-Epigallocatechin-3-gallate decreases osteoclastogenesis via modulation of RANKL and osteoprotegrin. Molecules, 24(1), [156]. https://doi.org/10.3390/molecules24010156