(−)-Epigallocatechin-3-gallate decreases osteoclastogenesis via modulation of RANKL and osteoprotegrin

Shih Tse Chen, Lin Kang, Chau Zen Wang, Peng Ju Huang, Hsuan Ti Huang, Sung Yen Lin, Shih Hsiang Chou, Cheng Chang Lu, Po Chih Shen, Yi Shan Lin, Chung Hwan Chen

研究成果: Article

3 引文 (Scopus)

摘要

Osteoporosis is the second most common epidemiologic disease in the aging population worldwide. Previous studies have found that frequent tea drinkers have higher bone mineral density and less hip fracture. We previously found that (−)-epigallocatechin gallate (EGCG) (20–100 µmol/L) significantly suppressed receptor activator of nuclear factor-kB ligand (RANKL)-induced osteoclastogenesis and pit formation via inhibiting NF-κB transcriptional activity and nuclear transport of NF-κB in RAW 264.7 cells and murine primary bone marrow macrophage cells. The most important regulation in osteoclastogenesis is the receptor activator of nuclear factor-kB/ RANKL/osteoprotegrin (RANK/RANKL/OPG) pathway. In this study, we used the coculture of RAW 264.7 cells and the feeder cells, ST2, to evaluate how EGCG regulated the RANK/RANKL/OPG pathway in RAW 264.7 cells and ST2 cells. We found EGCG decreased the RANKL/OPG ratio in both mRNA expression and secretory protein levels and eventually decreased osteoclastogenesis by TRAP (+) stain osteoclasts and TRAP activity at low concentrations—1 and 10 µmol/L—via the RANK/RANKL/OPG pathway. The effective concentration can be easily achieved in daily tea consumption. Taken together, our results implicate that EGCG could be an important nutrient in modulating bone resorption.

原文English
文章編號156
期刊Molecules
24
發行號1
DOIs
出版狀態Published - 2019 一月 3

指紋

gallates
Cytoplasmic and Nuclear Receptors
Osteogenesis
Modulation
Ligands
modulation
Bone
ligands
cells
Tea
Feeder Cells
bones
Macrophages
Nutrients
osteoporosis
Minerals
feeders
macrophages
Coloring Agents
Cell Nucleus Active Transport

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

引用此文

Chen, Shih Tse ; Kang, Lin ; Wang, Chau Zen ; Huang, Peng Ju ; Huang, Hsuan Ti ; Lin, Sung Yen ; Chou, Shih Hsiang ; Lu, Cheng Chang ; Shen, Po Chih ; Lin, Yi Shan ; Chen, Chung Hwan. / (−)-Epigallocatechin-3-gallate decreases osteoclastogenesis via modulation of RANKL and osteoprotegrin. 於: Molecules. 2019 ; 卷 24, 編號 1.
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title = "(−)-Epigallocatechin-3-gallate decreases osteoclastogenesis via modulation of RANKL and osteoprotegrin",
abstract = "Osteoporosis is the second most common epidemiologic disease in the aging population worldwide. Previous studies have found that frequent tea drinkers have higher bone mineral density and less hip fracture. We previously found that (−)-epigallocatechin gallate (EGCG) (20–100 µmol/L) significantly suppressed receptor activator of nuclear factor-kB ligand (RANKL)-induced osteoclastogenesis and pit formation via inhibiting NF-κB transcriptional activity and nuclear transport of NF-κB in RAW 264.7 cells and murine primary bone marrow macrophage cells. The most important regulation in osteoclastogenesis is the receptor activator of nuclear factor-kB/ RANKL/osteoprotegrin (RANK/RANKL/OPG) pathway. In this study, we used the coculture of RAW 264.7 cells and the feeder cells, ST2, to evaluate how EGCG regulated the RANK/RANKL/OPG pathway in RAW 264.7 cells and ST2 cells. We found EGCG decreased the RANKL/OPG ratio in both mRNA expression and secretory protein levels and eventually decreased osteoclastogenesis by TRAP (+) stain osteoclasts and TRAP activity at low concentrations—1 and 10 µmol/L—via the RANK/RANKL/OPG pathway. The effective concentration can be easily achieved in daily tea consumption. Taken together, our results implicate that EGCG could be an important nutrient in modulating bone resorption.",
author = "Chen, {Shih Tse} and Lin Kang and Wang, {Chau Zen} and Huang, {Peng Ju} and Huang, {Hsuan Ti} and Lin, {Sung Yen} and Chou, {Shih Hsiang} and Lu, {Cheng Chang} and Shen, {Po Chih} and Lin, {Yi Shan} and Chen, {Chung Hwan}",
year = "2019",
month = "1",
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doi = "10.3390/molecules24010156",
language = "English",
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Chen, ST, Kang, L, Wang, CZ, Huang, PJ, Huang, HT, Lin, SY, Chou, SH, Lu, CC, Shen, PC, Lin, YS & Chen, CH 2019, '(−)-Epigallocatechin-3-gallate decreases osteoclastogenesis via modulation of RANKL and osteoprotegrin', Molecules, 卷 24, 編號 1, 156. https://doi.org/10.3390/molecules24010156

(−)-Epigallocatechin-3-gallate decreases osteoclastogenesis via modulation of RANKL and osteoprotegrin. / Chen, Shih Tse; Kang, Lin; Wang, Chau Zen; Huang, Peng Ju; Huang, Hsuan Ti; Lin, Sung Yen; Chou, Shih Hsiang; Lu, Cheng Chang; Shen, Po Chih; Lin, Yi Shan; Chen, Chung Hwan.

於: Molecules, 卷 24, 編號 1, 156, 03.01.2019.

研究成果: Article

TY - JOUR

T1 - (−)-Epigallocatechin-3-gallate decreases osteoclastogenesis via modulation of RANKL and osteoprotegrin

AU - Chen, Shih Tse

AU - Kang, Lin

AU - Wang, Chau Zen

AU - Huang, Peng Ju

AU - Huang, Hsuan Ti

AU - Lin, Sung Yen

AU - Chou, Shih Hsiang

AU - Lu, Cheng Chang

AU - Shen, Po Chih

AU - Lin, Yi Shan

AU - Chen, Chung Hwan

PY - 2019/1/3

Y1 - 2019/1/3

N2 - Osteoporosis is the second most common epidemiologic disease in the aging population worldwide. Previous studies have found that frequent tea drinkers have higher bone mineral density and less hip fracture. We previously found that (−)-epigallocatechin gallate (EGCG) (20–100 µmol/L) significantly suppressed receptor activator of nuclear factor-kB ligand (RANKL)-induced osteoclastogenesis and pit formation via inhibiting NF-κB transcriptional activity and nuclear transport of NF-κB in RAW 264.7 cells and murine primary bone marrow macrophage cells. The most important regulation in osteoclastogenesis is the receptor activator of nuclear factor-kB/ RANKL/osteoprotegrin (RANK/RANKL/OPG) pathway. In this study, we used the coculture of RAW 264.7 cells and the feeder cells, ST2, to evaluate how EGCG regulated the RANK/RANKL/OPG pathway in RAW 264.7 cells and ST2 cells. We found EGCG decreased the RANKL/OPG ratio in both mRNA expression and secretory protein levels and eventually decreased osteoclastogenesis by TRAP (+) stain osteoclasts and TRAP activity at low concentrations—1 and 10 µmol/L—via the RANK/RANKL/OPG pathway. The effective concentration can be easily achieved in daily tea consumption. Taken together, our results implicate that EGCG could be an important nutrient in modulating bone resorption.

AB - Osteoporosis is the second most common epidemiologic disease in the aging population worldwide. Previous studies have found that frequent tea drinkers have higher bone mineral density and less hip fracture. We previously found that (−)-epigallocatechin gallate (EGCG) (20–100 µmol/L) significantly suppressed receptor activator of nuclear factor-kB ligand (RANKL)-induced osteoclastogenesis and pit formation via inhibiting NF-κB transcriptional activity and nuclear transport of NF-κB in RAW 264.7 cells and murine primary bone marrow macrophage cells. The most important regulation in osteoclastogenesis is the receptor activator of nuclear factor-kB/ RANKL/osteoprotegrin (RANK/RANKL/OPG) pathway. In this study, we used the coculture of RAW 264.7 cells and the feeder cells, ST2, to evaluate how EGCG regulated the RANK/RANKL/OPG pathway in RAW 264.7 cells and ST2 cells. We found EGCG decreased the RANKL/OPG ratio in both mRNA expression and secretory protein levels and eventually decreased osteoclastogenesis by TRAP (+) stain osteoclasts and TRAP activity at low concentrations—1 and 10 µmol/L—via the RANK/RANKL/OPG pathway. The effective concentration can be easily achieved in daily tea consumption. Taken together, our results implicate that EGCG could be an important nutrient in modulating bone resorption.

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