(−)-Epigallocatechin-3-gallate (EGCG) enhances healing of femoral bone defect

Sung Yen Lin, Lin Kang, Jian Chih Chen, Chau Zen Wang, Han Hsiang Huang, Mon Juan Lee, Tsung Lin Cheng, Chi Fen Chang, Yi Shan Lin, Chung Hwan Chen

研究成果: Article

3 引文 (Scopus)

摘要

Background: Previously, we found that (−)-epigallocatechin-3-gallate (EGCG) enhanced osteogenic differentiation of murine bone marrow mesenchymal stem cells by increasing the mRNA expression of osteogenesis-related genes, alkaline phosphatase activity and eventually mineralization. We further found EGCG supplementation preserved bone mass and microarchitecture in female rats during estrogen deficiency in the proximal tibia and lumbar spine at least in part by increasing bone morphogenetic protein-2 (BMP2). BMP2 can enhance de novo bone formation. Purpose: In this study, we evaluate the effect of local EGCG application in de novo bone formation in bone defect healing. Methods: Twenty-four rats aged 4 months were weight-matched and randomly allocated to 2 groups: defect control with vehicle treatment (control) and defect with 10 µM EGCG treatment (EGCG). Daily vehicle and EGCG were applied locally by percutaneous local injection 2 days after defect creation for 2 weeks. Four weeks after treatment, animals were sacrificed for micro-computed tomography (μ-CT) and biomechanical analysis. Results: Local EGCG at femoral defect can enhance de novo bone formation by increasing bone volume and subsequently improve mechanical properties including max load, break point, stiffness, area under the max load curve, area under the break point curve and ultimate stress. Conclusions: Local EGCG may enhance bone defect healing via at least partly by the de novo bone formation of BMP-2.

原文English
頁(從 - 到)165-171
頁數7
期刊Phytomedicine
55
DOIs
出版狀態Published - 2019 三月 1

指紋

Thigh
Bone and Bones
Osteogenesis
Bone Morphogenetic Protein 2
epigallocatechin gallate
Tibia
Mesenchymal Stromal Cells
Area Under Curve
Alkaline Phosphatase
Estrogens
Spine
Therapeutics
Bone Marrow
Tomography
Weights and Measures
Control Groups
Messenger RNA
Injections
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

引用此文

Lin, S. Y., Kang, L., Chen, J. C., Wang, C. Z., Huang, H. H., Lee, M. J., ... Chen, C. H. (2019). (−)-Epigallocatechin-3-gallate (EGCG) enhances healing of femoral bone defect. Phytomedicine, 55, 165-171. https://doi.org/10.1016/j.phymed.2018.07.012
Lin, Sung Yen ; Kang, Lin ; Chen, Jian Chih ; Wang, Chau Zen ; Huang, Han Hsiang ; Lee, Mon Juan ; Cheng, Tsung Lin ; Chang, Chi Fen ; Lin, Yi Shan ; Chen, Chung Hwan. / (−)-Epigallocatechin-3-gallate (EGCG) enhances healing of femoral bone defect. 於: Phytomedicine. 2019 ; 卷 55. 頁 165-171.
@article{9714cf55b8654a878f08bc0071221fa8,
title = "(−)-Epigallocatechin-3-gallate (EGCG) enhances healing of femoral bone defect",
abstract = "Background: Previously, we found that (−)-epigallocatechin-3-gallate (EGCG) enhanced osteogenic differentiation of murine bone marrow mesenchymal stem cells by increasing the mRNA expression of osteogenesis-related genes, alkaline phosphatase activity and eventually mineralization. We further found EGCG supplementation preserved bone mass and microarchitecture in female rats during estrogen deficiency in the proximal tibia and lumbar spine at least in part by increasing bone morphogenetic protein-2 (BMP2). BMP2 can enhance de novo bone formation. Purpose: In this study, we evaluate the effect of local EGCG application in de novo bone formation in bone defect healing. Methods: Twenty-four rats aged 4 months were weight-matched and randomly allocated to 2 groups: defect control with vehicle treatment (control) and defect with 10 µM EGCG treatment (EGCG). Daily vehicle and EGCG were applied locally by percutaneous local injection 2 days after defect creation for 2 weeks. Four weeks after treatment, animals were sacrificed for micro-computed tomography (μ-CT) and biomechanical analysis. Results: Local EGCG at femoral defect can enhance de novo bone formation by increasing bone volume and subsequently improve mechanical properties including max load, break point, stiffness, area under the max load curve, area under the break point curve and ultimate stress. Conclusions: Local EGCG may enhance bone defect healing via at least partly by the de novo bone formation of BMP-2.",
author = "Lin, {Sung Yen} and Lin Kang and Chen, {Jian Chih} and Wang, {Chau Zen} and Huang, {Han Hsiang} and Lee, {Mon Juan} and Cheng, {Tsung Lin} and Chang, {Chi Fen} and Lin, {Yi Shan} and Chen, {Chung Hwan}",
year = "2019",
month = "3",
day = "1",
doi = "10.1016/j.phymed.2018.07.012",
language = "English",
volume = "55",
pages = "165--171",
journal = "Phytomedicine",
issn = "0944-7113",
publisher = "Urban und Fischer Verlag Jena",

}

Lin, SY, Kang, L, Chen, JC, Wang, CZ, Huang, HH, Lee, MJ, Cheng, TL, Chang, CF, Lin, YS & Chen, CH 2019, '(−)-Epigallocatechin-3-gallate (EGCG) enhances healing of femoral bone defect', Phytomedicine, 卷 55, 頁 165-171. https://doi.org/10.1016/j.phymed.2018.07.012

(−)-Epigallocatechin-3-gallate (EGCG) enhances healing of femoral bone defect. / Lin, Sung Yen; Kang, Lin; Chen, Jian Chih; Wang, Chau Zen; Huang, Han Hsiang; Lee, Mon Juan; Cheng, Tsung Lin; Chang, Chi Fen; Lin, Yi Shan; Chen, Chung Hwan.

於: Phytomedicine, 卷 55, 01.03.2019, p. 165-171.

研究成果: Article

TY - JOUR

T1 - (−)-Epigallocatechin-3-gallate (EGCG) enhances healing of femoral bone defect

AU - Lin, Sung Yen

AU - Kang, Lin

AU - Chen, Jian Chih

AU - Wang, Chau Zen

AU - Huang, Han Hsiang

AU - Lee, Mon Juan

AU - Cheng, Tsung Lin

AU - Chang, Chi Fen

AU - Lin, Yi Shan

AU - Chen, Chung Hwan

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Background: Previously, we found that (−)-epigallocatechin-3-gallate (EGCG) enhanced osteogenic differentiation of murine bone marrow mesenchymal stem cells by increasing the mRNA expression of osteogenesis-related genes, alkaline phosphatase activity and eventually mineralization. We further found EGCG supplementation preserved bone mass and microarchitecture in female rats during estrogen deficiency in the proximal tibia and lumbar spine at least in part by increasing bone morphogenetic protein-2 (BMP2). BMP2 can enhance de novo bone formation. Purpose: In this study, we evaluate the effect of local EGCG application in de novo bone formation in bone defect healing. Methods: Twenty-four rats aged 4 months were weight-matched and randomly allocated to 2 groups: defect control with vehicle treatment (control) and defect with 10 µM EGCG treatment (EGCG). Daily vehicle and EGCG were applied locally by percutaneous local injection 2 days after defect creation for 2 weeks. Four weeks after treatment, animals were sacrificed for micro-computed tomography (μ-CT) and biomechanical analysis. Results: Local EGCG at femoral defect can enhance de novo bone formation by increasing bone volume and subsequently improve mechanical properties including max load, break point, stiffness, area under the max load curve, area under the break point curve and ultimate stress. Conclusions: Local EGCG may enhance bone defect healing via at least partly by the de novo bone formation of BMP-2.

AB - Background: Previously, we found that (−)-epigallocatechin-3-gallate (EGCG) enhanced osteogenic differentiation of murine bone marrow mesenchymal stem cells by increasing the mRNA expression of osteogenesis-related genes, alkaline phosphatase activity and eventually mineralization. We further found EGCG supplementation preserved bone mass and microarchitecture in female rats during estrogen deficiency in the proximal tibia and lumbar spine at least in part by increasing bone morphogenetic protein-2 (BMP2). BMP2 can enhance de novo bone formation. Purpose: In this study, we evaluate the effect of local EGCG application in de novo bone formation in bone defect healing. Methods: Twenty-four rats aged 4 months were weight-matched and randomly allocated to 2 groups: defect control with vehicle treatment (control) and defect with 10 µM EGCG treatment (EGCG). Daily vehicle and EGCG were applied locally by percutaneous local injection 2 days after defect creation for 2 weeks. Four weeks after treatment, animals were sacrificed for micro-computed tomography (μ-CT) and biomechanical analysis. Results: Local EGCG at femoral defect can enhance de novo bone formation by increasing bone volume and subsequently improve mechanical properties including max load, break point, stiffness, area under the max load curve, area under the break point curve and ultimate stress. Conclusions: Local EGCG may enhance bone defect healing via at least partly by the de novo bone formation of BMP-2.

UR - http://www.scopus.com/inward/record.url?scp=85056200356&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056200356&partnerID=8YFLogxK

U2 - 10.1016/j.phymed.2018.07.012

DO - 10.1016/j.phymed.2018.07.012

M3 - Article

C2 - 30668426

AN - SCOPUS:85056200356

VL - 55

SP - 165

EP - 171

JO - Phytomedicine

JF - Phytomedicine

SN - 0944-7113

ER -