Epithelial-mesenchymal transition contributes to SWCNT-induced pulmonary fibrosis

Chih Ching Chang, Mei Ling Tsai, Hui Chun Huang, Chin Yu Chen, Shi Xun Dai

研究成果: Article

32 引文 斯高帕斯(Scopus)

摘要

Previous studies suggest that single-walled carbon nanotube (SWCNT) exposure causes pulmonary fibrosis. We investigated the contribution of epithelial-mesenchymal transition (EMT) during SWCNT-induced pulmonary fibrosis. C57BL6 female mice were intratracheally instilled with SWCNT at 80 μg/mouse for up to 56 days. SWCNT exposure caused pulmonary epithelial and mesenchymal injury, followed by granulomatous and fibrotic changes. Immunofluorescence staining demonstrated the increasing occurrence of epithelial-derived fibroblasts up to 42 days post-exposure. Flow cytometry analysis revealed that 42.60% of N-cadherin (N-cad)-positive fibroblasts were derived from pulmonary epithelial cells, and, in separate experiments, 30.68% of SPC positive cells were stained for N-cad at 42 days. These epithelial-derived fibroblasts were functional in collagen production. With the progression of fibrosis, there were increases in the number of hyperplastic epithelial cells stained positively for TGF-β/p-Smad2 or β-catenin. Therefore, EMT contributes significantly to fibroblast expansion. Aberrant activations of TGF-β/p-Smad2 and β-catenin are postulated to induce EMT during SWCNT-induced pathogenic fibrosis.

原文English
頁(從 - 到)600-610
頁數11
期刊Nanotoxicology
6
發行號6
DOIs
出版狀態Published - 2012 九月 1

    指紋

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Toxicology

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