Estrogen receptor expression affected by hypoxia inducible factor-1α in stromal cells from patients with endometriosis

Meng-Hsing Wu, Chun Wun Lu, Fong Ming Chang, Shaw-Jenq Tsai

研究成果: Article

20 引文 (Scopus)

摘要

Objective: Endometriosis is an estrogen-dependent disease. The aim of this study was to evaluate the different expression of estrogen receptors (ER) and its relation to hypoxia inducible factor-1α (HIF-1α) in stromal cells from women with endometriosis. Materials and Methods: Paired eutopic endometrial and ectopic endometriotic stromal cells were isolated from women with endometriosis while they underwent laparoscopy. The expression of ERα and ERβ was measured by reverse transcription-polymerase chain reaction and Western blot. Regulation of ER expression was evaluated by HIF-1α knockdown via short interference RNA. Results: The expression of ERβ was significantly increased in ectopic stromal cells. Treatment of endometrial stromal cells with hypoxia induced ERβ expression. Knockdown of HIF-1α abolished hypoxia-induced ERβ expression and increased ERα expression. Conclusion: The expression of ERβ is regulated by hypoxia. Results of this study will provide important information in the involvement of hypoxia factors in mediating estrogen action via different ER expression in endometriosis.

原文English
頁(從 - 到)50-54
頁數5
期刊Taiwanese Journal of Obstetrics and Gynecology
51
發行號1
DOIs
出版狀態Published - 2012 三月 1

指紋

Hypoxia-Inducible Factor 1
Endometriosis
Stromal Cells
Estrogen Receptors
Estrogens
Cell Hypoxia
RNA Interference
Laparoscopy
Reverse Transcription
Western Blotting

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynaecology

引用此文

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title = "Estrogen receptor expression affected by hypoxia inducible factor-1α in stromal cells from patients with endometriosis",
abstract = "Objective: Endometriosis is an estrogen-dependent disease. The aim of this study was to evaluate the different expression of estrogen receptors (ER) and its relation to hypoxia inducible factor-1α (HIF-1α) in stromal cells from women with endometriosis. Materials and Methods: Paired eutopic endometrial and ectopic endometriotic stromal cells were isolated from women with endometriosis while they underwent laparoscopy. The expression of ERα and ERβ was measured by reverse transcription-polymerase chain reaction and Western blot. Regulation of ER expression was evaluated by HIF-1α knockdown via short interference RNA. Results: The expression of ERβ was significantly increased in ectopic stromal cells. Treatment of endometrial stromal cells with hypoxia induced ERβ expression. Knockdown of HIF-1α abolished hypoxia-induced ERβ expression and increased ERα expression. Conclusion: The expression of ERβ is regulated by hypoxia. Results of this study will provide important information in the involvement of hypoxia factors in mediating estrogen action via different ER expression in endometriosis.",
author = "Meng-Hsing Wu and Lu, {Chun Wun} and Chang, {Fong Ming} and Shaw-Jenq Tsai",
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T1 - Estrogen receptor expression affected by hypoxia inducible factor-1α in stromal cells from patients with endometriosis

AU - Wu, Meng-Hsing

AU - Lu, Chun Wun

AU - Chang, Fong Ming

AU - Tsai, Shaw-Jenq

PY - 2012/3/1

Y1 - 2012/3/1

N2 - Objective: Endometriosis is an estrogen-dependent disease. The aim of this study was to evaluate the different expression of estrogen receptors (ER) and its relation to hypoxia inducible factor-1α (HIF-1α) in stromal cells from women with endometriosis. Materials and Methods: Paired eutopic endometrial and ectopic endometriotic stromal cells were isolated from women with endometriosis while they underwent laparoscopy. The expression of ERα and ERβ was measured by reverse transcription-polymerase chain reaction and Western blot. Regulation of ER expression was evaluated by HIF-1α knockdown via short interference RNA. Results: The expression of ERβ was significantly increased in ectopic stromal cells. Treatment of endometrial stromal cells with hypoxia induced ERβ expression. Knockdown of HIF-1α abolished hypoxia-induced ERβ expression and increased ERα expression. Conclusion: The expression of ERβ is regulated by hypoxia. Results of this study will provide important information in the involvement of hypoxia factors in mediating estrogen action via different ER expression in endometriosis.

AB - Objective: Endometriosis is an estrogen-dependent disease. The aim of this study was to evaluate the different expression of estrogen receptors (ER) and its relation to hypoxia inducible factor-1α (HIF-1α) in stromal cells from women with endometriosis. Materials and Methods: Paired eutopic endometrial and ectopic endometriotic stromal cells were isolated from women with endometriosis while they underwent laparoscopy. The expression of ERα and ERβ was measured by reverse transcription-polymerase chain reaction and Western blot. Regulation of ER expression was evaluated by HIF-1α knockdown via short interference RNA. Results: The expression of ERβ was significantly increased in ectopic stromal cells. Treatment of endometrial stromal cells with hypoxia induced ERβ expression. Knockdown of HIF-1α abolished hypoxia-induced ERβ expression and increased ERα expression. Conclusion: The expression of ERβ is regulated by hypoxia. Results of this study will provide important information in the involvement of hypoxia factors in mediating estrogen action via different ER expression in endometriosis.

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