Evaluating virulence and pathogenesis of Aeromonas Infection in a Caenorhabditis elegans model

研究成果: Article

摘要

The human pathogen Aeromonas has been clinically shown to cause gastroenteritis, wound infections, septicemia, and urinary tract infections. Most human diseases have been reported to be associated with four species of bacteria: Aeromonas dhakensis, Aeromonas hydrophila, Aeromonas veronii, and Aeromonas caviae. The model organism Caenorhabditis elegans is a bacterivore that provides an excellent infection model by which to study the bacterial pathogenesis of Aeromonas. Here, we introduce three different experiments to study Aeromonas infection using a C. elegans model, including survival, liquid toxicity, and muscle necrosis assays. The results of the three methods determining the virulence of Aeromonas were consistent. A. dhakensis was shown to be the most toxic among the 4 major Aeromonas species causing clinical infections. These methods are shown to be a convenient way to evaluate the toxicity among and within Aeromonas species and contribute to our understanding of the pathogenesis of Aeromonas infection.

原文English
文章編號e58768
期刊Journal of Visualized Experiments
2018
發行號142
DOIs
出版狀態Published - 2018 十二月

指紋

Aeromonas
Caenorhabditis elegans
Toxicity
Virulence
Poisons
Pathogens
Infection
Muscle
Assays
Bacteria
Liquids
Aeromonas caviae
Experiments
Aeromonas hydrophila
Gastroenteritis
Wound Infection
Urinary Tract Infections
Sepsis
Necrosis
Muscles

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

引用此文

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