Evaluation of a mycobacterium avium subsp. paratuberculosis leuD mutant as a vaccine candidate against challenge in a caprine model

Syed M. Faisal, Jenn Wei Chen, Falong Yan, Tsai Tzu Chen, Nicodemus M. Useh, Weiwei Yan, Shanguang Guo, Shih Jon Wang, Amy L. Glaser, Sean P. McDonough, Bhupinder Singh, William C. Davis, Bruce L. Akey, Yung Fu Chang

研究成果: Article

19 引文 斯高帕斯(Scopus)

摘要

Johne's disease (JD) is prevalent worldwide and has a significant impact on the global agricultural economy. In the present study, we evaluated the protective efficacy of a leuD (leud) mutant and gained insight into differential immune responses after challenge with virulent M. avium subsp. paratuberculosis in a caprine colonization model. The immune response and protective efficacy were compared with those of the killed vaccine Mycopar. In vitro stimulation of peripheral blood mononuclear cells with johnin purified protein derivative showed that Mycopar and γleuD generated similar levels of gamma interferon (IFN-γ) but significantly higher levels than unvaccinated and challenged phosphate-buffered saline controls. However, only with γleuD was the IFN-γ response maintained. Flow cytometric analysis showed that the increase in IFN-γ correlated with proliferation and activation (increased expression of CD25) of CD4, CD8, and γσT cells, but this response was significantly higher in δleuD-vaccinated animals at some time points after challenge. Both Mycopar and δleuD vaccines upregulated Th1/proinflammatory and Th17 cytokines and downregulated Th2/anti-inflammatory and regulatory cytokines at similar levels at almost all time points. However, significantly higher levels of IFN-γ (at weeks 26 and 30), interleukin-2 (IL-2; week 18), IL-1b (weeks 14 and 22), IL-17 (weeks 18 and 22), and IL-23 (week 18) and a significantly lower level of IL-10 (weeks 14 and 18) and transforming growth factor β (week 18) were detected in the δleuD-vaccinated group. Most importantly, δleuD elicited an immune response that significantly limited colonization of tissues compared to Mycopar upon challenge with wild-type M. avium subsp. paratuberculosis. In conclusion, the δleuD mutant is a promising vaccine candidate for development of a live attenuated vaccine for JD in ruminants.

原文English
頁(從 - 到)572-581
頁數10
期刊Clinical and Vaccine Immunology
20
發行號4
DOIs
出版狀態Published - 2013 四月

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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    Faisal, S. M., Chen, J. W., Yan, F., Chen, T. T., Useh, N. M., Yan, W., Guo, S., Wang, S. J., Glaser, A. L., McDonough, S. P., Singh, B., Davis, W. C., Akey, B. L., & Chang, Y. F. (2013). Evaluation of a mycobacterium avium subsp. paratuberculosis leuD mutant as a vaccine candidate against challenge in a caprine model. Clinical and Vaccine Immunology, 20(4), 572-581. https://doi.org/10.1128/CVI.00653-12