Evaluation of chloroplast DNA markers for distinguishing Phalaenopsis species

Jhong Yi Lin, Bo Yen Lin, Ching Dong Chang, Sin Chung Liao, Yu Chang Liu, Wen Luan Wu, Ching Chun Chang

研究成果: Article同行評審

13 引文 斯高帕斯(Scopus)

摘要

Comparative analysis of chloroplast DNA (cpDNA) between two endemic moth orchids in Taiwan, Phalaenopsis aphrodite subsp. formosana and P. equestris, previously revealed many evolutionary hotspots. In this study, we explored 27 pre-screened cpDNA regions to evaluate molecular markers for distinguishing Phalaenopsis species. Fifteen cpDNA markers were highly variable among moth orchids, with polymorphic information contents ≥8.0. The rps16-. trnQ marker showed the best discrimatory power which 15 endemic moth orchids could be successfully separated into 13 groups. From the sequences of the seven selected cpDNA regions, the rps16-. trnQ intergenic spacer was the best DNA barcode which 19 endemic moth orchids could be completely distinguished. The petN-. psbM intergenic spacer could be better used as a DNA barcode to differentiate P. aphrodite subsp. formosana and P. amabilis. Additionally, the inheritance mode of cpDNA in moth orchids was maternally predominated. Phylogenetic analysis revealed non-monophyletic relationships below the Phalaenopsis genus level. In summary, we have revealed a set of cpDNA markers that could be used for identification and phylogenetic study of Phalaenopsis orchids.

原文English
頁(從 - 到)302-310
頁數9
期刊Scientia Horticulturae
192
DOIs
出版狀態Published - 2015 8月 1

All Science Journal Classification (ASJC) codes

  • 園藝科學

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