Evaluation of macrolide resistance and enhanced molecular typing of Treponema pallidum in patients with syphilis in taiwan: A prospective multicenter study

Hsiu Wu, Sui Yuan Chang, Nan Yao Lee, Wen Chi Huang, Bing Ru Wu, Chia Jui Yang, Shiou Haur Liang, Chen Hsiang Lee, Wen Chien Ko, Hsi Hsun Lin, Yen Hsu Chen, Wen Chun Liu, Yi Ching Su, Chia Yin Hsieh, Pei Ying Wu, Chien Ching Hung

研究成果: Article

37 引文 斯高帕斯(Scopus)

摘要

Studies of macrolide resistance mutations and molecular typing using the newly proposed enhanced typing system for Treponema pallidum isolates obtained from HIV-infected patients in the Asia-Pacific region are scarce. Between September 2009 and December 2011, we conducted a survey to detect T. pallidum using a PCR assay using clinical specimens from patients with syphilis at six major designated hospitals for HIV care in Taiwan. The T. pallidum strains were genotyped by following the enhanced molecular typing methodology, which analyzed the number of 60-bp repeats in the acidic repeat protein (arp) gene, T. pallidum repeat (tpr) polymorphism, and the sequence of base pairs 131 to 215 in the tp0548 open reading frame of T. pallidum. Detection of A2058G and A2059G point mutations in the T. pallidum 23S rRNA was performed with the use of restriction fragment length polymorphism (RFLP). During the 2-year study period, 211 clinical specimens were obtained from 136 patients with syphilis. T. pallidum DNA was isolated from 105 (49.8%) of the specimens, with swab specimens obtained from chancres having the highest yield rate (63.2%), followed by plasma (49.4%), serum (35.7%), and cerebrospinal fluid or vitreous fluid (18.2%) specimens. Among the 40 fully typed specimens, 11 subtypes of T. pallidum were identified. Subtype 14f/f (18 isolates) was the most common isolates, followed by 14f/c (3), 14b/c (3), and 14k/f (3). Among the isolates examined for macrolide resistance, none had the A2058G or A2059G mutation. In conclusion, we found that type 14 f/f was the most common T. pallidum strain in this multicenter study on syphilis in Taiwan and that none of the isolates exhibited 23S rRNA mutations causing resistance to macrolides.

原文English
頁(從 - 到)2299-2304
頁數6
期刊Journal of Clinical Microbiology
50
發行號7
DOIs
出版狀態Published - 2012 七月

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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    Wu, H., Chang, S. Y., Lee, N. Y., Huang, W. C., Wu, B. R., Yang, C. J., Liang, S. H., Lee, C. H., Ko, W. C., Lin, H. H., Chen, Y. H., Liu, W. C., Su, Y. C., Hsieh, C. Y., Wu, P. Y., & Hung, C. C. (2012). Evaluation of macrolide resistance and enhanced molecular typing of Treponema pallidum in patients with syphilis in taiwan: A prospective multicenter study. Journal of Clinical Microbiology, 50(7), 2299-2304. https://doi.org/10.1128/JCM.00341-12