Evidence for the Inhibition by Temozolomide, an Imidazotetrazine Family Alkylator, of Intermediate-Conductance Ca 2+ -Activated K + Channels in Glioma Cells

Poh Shiow Yeh, Shyh Jong Wu, Te Yu Hung, Yan Ming Huang, Chia Wei Hsu, Chun-I Sze, Yi Jung Hsieh, Chin-Wei Huang, Sheng-Nan Wu

研究成果: Article

5 引文 (Scopus)

摘要

Background: Temozolomide (TMZ), an oral alkylator of the imidazotetrazine family, is used to treat glioma. Whether this drug has any ionic effects in glioma cells remains largely unclear. Methods: With the aid of patch-clamp technology, we investigated the effects of TMZ on the ionic currents in U373 glioma cells. The mRNA expression of KCNN4 (K Ca 3.1) in U373 glioma cells and TMZ's effect on K + currents in these KCNN4 siRNA-transfected U373 cells were investigated. Results: In whole-cell recordings, TMZ decreased the amplitude of voltage-dependent K + currents (I K ) in U373 cells. TMZ-induced I K inhibition was reversed by ionomycin or 1-ethyl-2-benzimidazolinone (1-EBIO). In cell-attached configuration, TMZ concentration-dependently reduced the activity of intermediate-conductance Ca 2+ -activated K + (IK Ca ) channels with an IC 50 value of 9.2 μM. Chlorzoxazone or 1-EBIO counteracted the TMZ-induced inhibition of IK Ca channels. Although TMZ was unable to modify single-channel conductance, its inhibition of IK Ca channels was weakly voltage-dependent and accompanied by a significant prolongation in the slow component of mean closed time. However, neither large-conductance Ca 2+ -activated (BK Ca ) nor inwardly rectifying K + (Kir) channels were affected by TMZ. In current-clamp mode, TMZ depolarized the cell membrane and 1-EBIO reversed TMZ-induced depolarization. TMZ had no effect on I K in KCNN4 siRNA-transfected U373 cells. Conclusion: In addition to the DNA damage it does, its inhibitory effect on IKCa channels accompanied by membrane depolarization could be an important mechanism underlying TMZ-induced antineoplastic actions.

原文English
頁(從 - 到)1727-1742
頁數16
期刊Cellular Physiology and Biochemistry
38
發行號5
DOIs
出版狀態Published - 2016 一月 1

指紋

temozolomide
Alkylating Agents
Glioma
Small Interfering RNA
Chlorzoxazone
Inwardly Rectifying Potassium Channel

All Science Journal Classification (ASJC) codes

  • Physiology

引用此文

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title = "Evidence for the Inhibition by Temozolomide, an Imidazotetrazine Family Alkylator, of Intermediate-Conductance Ca 2+ -Activated K + Channels in Glioma Cells",
abstract = "Background: Temozolomide (TMZ), an oral alkylator of the imidazotetrazine family, is used to treat glioma. Whether this drug has any ionic effects in glioma cells remains largely unclear. Methods: With the aid of patch-clamp technology, we investigated the effects of TMZ on the ionic currents in U373 glioma cells. The mRNA expression of KCNN4 (K Ca 3.1) in U373 glioma cells and TMZ's effect on K + currents in these KCNN4 siRNA-transfected U373 cells were investigated. Results: In whole-cell recordings, TMZ decreased the amplitude of voltage-dependent K + currents (I K ) in U373 cells. TMZ-induced I K inhibition was reversed by ionomycin or 1-ethyl-2-benzimidazolinone (1-EBIO). In cell-attached configuration, TMZ concentration-dependently reduced the activity of intermediate-conductance Ca 2+ -activated K + (IK Ca ) channels with an IC 50 value of 9.2 μM. Chlorzoxazone or 1-EBIO counteracted the TMZ-induced inhibition of IK Ca channels. Although TMZ was unable to modify single-channel conductance, its inhibition of IK Ca channels was weakly voltage-dependent and accompanied by a significant prolongation in the slow component of mean closed time. However, neither large-conductance Ca 2+ -activated (BK Ca ) nor inwardly rectifying K + (Kir) channels were affected by TMZ. In current-clamp mode, TMZ depolarized the cell membrane and 1-EBIO reversed TMZ-induced depolarization. TMZ had no effect on I K in KCNN4 siRNA-transfected U373 cells. Conclusion: In addition to the DNA damage it does, its inhibitory effect on IKCa channels accompanied by membrane depolarization could be an important mechanism underlying TMZ-induced antineoplastic actions.",
author = "Yeh, {Poh Shiow} and Wu, {Shyh Jong} and Hung, {Te Yu} and Huang, {Yan Ming} and Hsu, {Chia Wei} and Chun-I Sze and Hsieh, {Yi Jung} and Chin-Wei Huang and Sheng-Nan Wu",
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Evidence for the Inhibition by Temozolomide, an Imidazotetrazine Family Alkylator, of Intermediate-Conductance Ca 2+ -Activated K + Channels in Glioma Cells . / Yeh, Poh Shiow; Wu, Shyh Jong; Hung, Te Yu; Huang, Yan Ming; Hsu, Chia Wei; Sze, Chun-I; Hsieh, Yi Jung; Huang, Chin-Wei; Wu, Sheng-Nan.

於: Cellular Physiology and Biochemistry, 卷 38, 編號 5, 01.01.2016, p. 1727-1742.

研究成果: Article

TY - JOUR

T1 - Evidence for the Inhibition by Temozolomide, an Imidazotetrazine Family Alkylator, of Intermediate-Conductance Ca 2+ -Activated K + Channels in Glioma Cells

AU - Yeh, Poh Shiow

AU - Wu, Shyh Jong

AU - Hung, Te Yu

AU - Huang, Yan Ming

AU - Hsu, Chia Wei

AU - Sze, Chun-I

AU - Hsieh, Yi Jung

AU - Huang, Chin-Wei

AU - Wu, Sheng-Nan

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Background: Temozolomide (TMZ), an oral alkylator of the imidazotetrazine family, is used to treat glioma. Whether this drug has any ionic effects in glioma cells remains largely unclear. Methods: With the aid of patch-clamp technology, we investigated the effects of TMZ on the ionic currents in U373 glioma cells. The mRNA expression of KCNN4 (K Ca 3.1) in U373 glioma cells and TMZ's effect on K + currents in these KCNN4 siRNA-transfected U373 cells were investigated. Results: In whole-cell recordings, TMZ decreased the amplitude of voltage-dependent K + currents (I K ) in U373 cells. TMZ-induced I K inhibition was reversed by ionomycin or 1-ethyl-2-benzimidazolinone (1-EBIO). In cell-attached configuration, TMZ concentration-dependently reduced the activity of intermediate-conductance Ca 2+ -activated K + (IK Ca ) channels with an IC 50 value of 9.2 μM. Chlorzoxazone or 1-EBIO counteracted the TMZ-induced inhibition of IK Ca channels. Although TMZ was unable to modify single-channel conductance, its inhibition of IK Ca channels was weakly voltage-dependent and accompanied by a significant prolongation in the slow component of mean closed time. However, neither large-conductance Ca 2+ -activated (BK Ca ) nor inwardly rectifying K + (Kir) channels were affected by TMZ. In current-clamp mode, TMZ depolarized the cell membrane and 1-EBIO reversed TMZ-induced depolarization. TMZ had no effect on I K in KCNN4 siRNA-transfected U373 cells. Conclusion: In addition to the DNA damage it does, its inhibitory effect on IKCa channels accompanied by membrane depolarization could be an important mechanism underlying TMZ-induced antineoplastic actions.

AB - Background: Temozolomide (TMZ), an oral alkylator of the imidazotetrazine family, is used to treat glioma. Whether this drug has any ionic effects in glioma cells remains largely unclear. Methods: With the aid of patch-clamp technology, we investigated the effects of TMZ on the ionic currents in U373 glioma cells. The mRNA expression of KCNN4 (K Ca 3.1) in U373 glioma cells and TMZ's effect on K + currents in these KCNN4 siRNA-transfected U373 cells were investigated. Results: In whole-cell recordings, TMZ decreased the amplitude of voltage-dependent K + currents (I K ) in U373 cells. TMZ-induced I K inhibition was reversed by ionomycin or 1-ethyl-2-benzimidazolinone (1-EBIO). In cell-attached configuration, TMZ concentration-dependently reduced the activity of intermediate-conductance Ca 2+ -activated K + (IK Ca ) channels with an IC 50 value of 9.2 μM. Chlorzoxazone or 1-EBIO counteracted the TMZ-induced inhibition of IK Ca channels. Although TMZ was unable to modify single-channel conductance, its inhibition of IK Ca channels was weakly voltage-dependent and accompanied by a significant prolongation in the slow component of mean closed time. However, neither large-conductance Ca 2+ -activated (BK Ca ) nor inwardly rectifying K + (Kir) channels were affected by TMZ. In current-clamp mode, TMZ depolarized the cell membrane and 1-EBIO reversed TMZ-induced depolarization. TMZ had no effect on I K in KCNN4 siRNA-transfected U373 cells. Conclusion: In addition to the DNA damage it does, its inhibitory effect on IKCa channels accompanied by membrane depolarization could be an important mechanism underlying TMZ-induced antineoplastic actions.

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