TY - JOUR
T1 - Evidence of adenosine 5'-triphosphate release from nerve and P2x- purinoceptor mediated contraction during electrical stimulation of rat urinary bladder smooth muscle
AU - Tong, Yat Ching
AU - Hung, Ying Cho
AU - Shinozuka, Kazumasa
AU - Kunitomo, Masaru
AU - Cheng, Juei Tang
N1 - Funding Information:
This study was supported by Grants from the National Science Council, Taiwan, Republic of China (NSC83-0412-B-006-043 and NSC85-2331-B-006-103), a Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Science, Sports and Culture, Japan and a SRF Grant for Biomedical Research, Japan.
PY - 1997/11
Y1 - 1997/11
N2 - Purpose: We provided direct evidence for the existence of purinergic innervation in the rat urinary bladder. Materials and Methods: The non- adrenergic non-cholinergic (NANC) innervation was studied in 4-month-old Wistar rats. Electric-field stimulation (EFS) of the detrusor muscle strips in the presence of four autonomic blockers (atropine 10-6 M, guanethidine 10-6 M, phentolamine 10-6 M and propranolol 10-6 M) showed NANC contractions accounted for about 50% of the maximum contractile response. The adenyl purines released from nerves by EFS were detected by HPLC after conversion to ethenopurines. The amount of total purine released was frequency-dependent and could be totally suppressed by tetradotoxin (10- 6 M). The amount of ATP released was significantly greater than those for ADP, AMP and adenosine (p <0.05, n = 4). Desensitization induced by α, β- MeATP (10-6 to 10-4 M), a P2x receptor agonist, reduced the NANC contraction. In addition, the NANC contraction was also abolished by P2 receptor blocker suramin (10-4 to 10-3 M) and P2x receptor blocker PPADS (10-5 to 10-4 M.). Conclusion: The results of the present study give evidence to support purinergic nerve-mediated bladder smooth muscle contractions in the rat. Among the purine nucleotides, ATP is the dominant purinergic neurotransmitter released and P2x receptor activation is responsible for the NANC contractile response.
AB - Purpose: We provided direct evidence for the existence of purinergic innervation in the rat urinary bladder. Materials and Methods: The non- adrenergic non-cholinergic (NANC) innervation was studied in 4-month-old Wistar rats. Electric-field stimulation (EFS) of the detrusor muscle strips in the presence of four autonomic blockers (atropine 10-6 M, guanethidine 10-6 M, phentolamine 10-6 M and propranolol 10-6 M) showed NANC contractions accounted for about 50% of the maximum contractile response. The adenyl purines released from nerves by EFS were detected by HPLC after conversion to ethenopurines. The amount of total purine released was frequency-dependent and could be totally suppressed by tetradotoxin (10- 6 M). The amount of ATP released was significantly greater than those for ADP, AMP and adenosine (p <0.05, n = 4). Desensitization induced by α, β- MeATP (10-6 to 10-4 M), a P2x receptor agonist, reduced the NANC contraction. In addition, the NANC contraction was also abolished by P2 receptor blocker suramin (10-4 to 10-3 M) and P2x receptor blocker PPADS (10-5 to 10-4 M.). Conclusion: The results of the present study give evidence to support purinergic nerve-mediated bladder smooth muscle contractions in the rat. Among the purine nucleotides, ATP is the dominant purinergic neurotransmitter released and P2x receptor activation is responsible for the NANC contractile response.
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U2 - 10.1016/S0022-5347(01)64196-X
DO - 10.1016/S0022-5347(01)64196-X
M3 - Article
C2 - 9334652
AN - SCOPUS:0030755089
SN - 0022-5347
VL - 158
SP - 1973
EP - 1977
JO - Journal of Urology
JF - Journal of Urology
IS - 5
ER -