Exfoliated tumor cells in bile as a promising indicator of disease status in cholangiocarcinoma

Priya Gopinathan, Tsung Han Lu, Nai Jung Chiang, Chien Jui Huang, Hsui Chi Tu, Shang Cheng Hung, Yan Shen Shan, Gwo Bin Lee

研究成果: Article同行評審

1 引文 斯高帕斯(Scopus)


Cholangiocarcinoma (CCA) is highly metastatic, extremely difficult to diagnose and is consequently characterized by a low 5-year survival rate. Herein we investigated the potential of using exfoliated tumor cells (ETCs) in bile to serve as an early-warning CCA metastasis indicator. ETCs were isolated from bile using a CCA-specific aptamer conjugated to magnetic beads and identified via immunostaining (CK17+CK7+Hoechst+). A conventional protein biomarker, epithelial cell adhesion molecule (EpCAM), was used for comparison. Circulating tumor cell (CTC) isolation in blood was achieved by a microfluidic system from samples from five CCA patients undergoing chemotherapy, and the enumeration results of ETCs and CTCs were compared with diagnoses derived from computed tomography scans. The CCA-specific aptamer-conjugated magnetic beads effectively bound ETCs from bile samples of 40/40 CCA patients (>1 ETC in 3 ml of bile), and the ETC and CTC count data from patients corresponded well with cancer progression and tumor size. ETCs in bile could serve as promising indicators of disease status in early-advanced stage CCA while CTCs in blood could be representative of late-advanced stage CCA. The developed technique could consequently be a powerful tool in CCA diagnostics and prognostics.

期刊Sensors and Actuators B: Chemical
出版狀態Published - 2021 11月 1

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 儀器
  • 凝聚態物理學
  • 表面、塗料和薄膜
  • 金屬和合金
  • 電氣與電子工程
  • 材料化學


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