Expression and characterization of recombinant human cytochrome c in E. coli

Wen Yih Jeng, Chiu Yueh Chen, Hsien Chang Chang, Woei Jer Chuang

研究成果: Article同行評審

36 引文 斯高帕斯(Scopus)

摘要

Cytochrome c is a heme protein involved in electron transfer, cell apoptosis, and diseases associated with oxidative stress. Here we expressed human cytochrome c in E. coli and purified it to homogeneity with a yield of 10-15 mg/L. The redox potential of recombinant human cytochrome c was 0.246 V which was measured by cyclic voltammetry. This is similar to that of horse cytochrome c with a value of 0.249 V. The sequential assignment and structural analysis of recombinant human ferrocytochrome c were obtained using multidimensional NMR spectroscopy. On the basis of our NMR studies, the recombinant human cytochrome c produced in E. coli exhibits the same tertiary fold as horse cytochrome c. These results provide evidence that human cytochrome c expressed in E. coli possesses a similar function and structure to that of the horse protein. It is known that cytochrome c plays a role in many human diseases. This study serves as the basis for gaining insight into human diseases by exploring structure and function relationships of cytochrome c to its interacting proteins.

原文English
頁(從 - 到)423-431
頁數9
期刊Journal of Bioenergetics and Biomembranes
34
發行號6
DOIs
出版狀態Published - 2002 12月

All Science Journal Classification (ASJC) codes

  • 生理學
  • 細胞生物學

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