Expression and non-chromatographic purification of 1,3-propanediol oxidoreductase in Escherichia coli

Wei Li, I. Son Ng, Baishan Fang, Guangya Zhang

研究成果: Article同行評審

1 引文 斯高帕斯(Scopus)

摘要

The gene dhaT from Klebsiella pneumoniae encodes 1,3-propanediol oxidoreductase (PDOR). Thermally responsive elastin-like polypeptides (ELPs) was used as a fusion tag to purify the proteins (PDOR). The ELP gene was attached to dhaT and ligated into the pET-22b vector. Different NaCl concentrations were employed to decrease the transition temperature (Tt) which was diminished as salt concentration increased. The optimal final concentration of NaCl was 1 M and the corresponding Tt was 39.5°C. Enzymatic assays were determined via every step for purification of fusion PDOR. PDOR showed good stability during the purification process, the specific activity in the first and second round of inverse transition cycling (ITC) was 276.1 ± 13.3 and 213.3 ± 10.8 U/mg, respectively. The ELPs fusion PDOR was superior to histidine tagged PDOR in both yield and activity after the purification.

原文English
期刊Electronic Journal of Biotechnology
14
發行號6
DOIs
出版狀態Published - 2011 11月 15

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 應用微生物與生物技術

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