Expression of the carbohydrate recognition domain of lung surfactant protein d and demonstration of its binding to lipopolysaccharides of gram-negative bacteria

Boon Leong Lim, Jiu Yao Wang, Uffe Holmskov, Hans Jürgen Hoppe, Kenneth B.M. Reid

研究成果: Article

99 引文 斯高帕斯(Scopus)

摘要

Surfactant protein D is a collagenous C-type lectin (collectin) that is found almost exclusively in the lung. A recombinant protein, composed of the neck-region and the carbohydrate binding domain of bovine lung surfactant protein D, has been overexpressed in E. coli. The recombinant protein showed the same sugar binding specificity as the native protein and was able to bind to the lipopolysaccharides of several strains of Gram-negative bacteria, such as Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli, which are known to cause lung infections. This binding was calcium-dependent and was inhibited by maltose. Native bovine surfactant protein D was also shown to be able to bind to these lipopolysaccharides in the same manner.

原文English
頁(從 - 到)1674-1680
頁數7
期刊Biochemical and Biophysical Research Communications
202
發行號3
DOIs
出版狀態Published - 1994 八月 15

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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