Facile preparation of polydiacetylene-based uniform porous fluorescent microspheres for potential immunoassay applications

Wei Zhang, Yun Chen, Ya Shao, Li Juan Fan

研究成果: Article同行評審

12 引文 斯高帕斯(Scopus)

摘要

Fluorescent microspheres are prepared by attaching self-assembled polydiacetylene (PDA) vesicles with carboxyl side groups onto the substrate amino-modified poly(glycidylmethacrylate) (APGMA) microspheres. The characterization by SEM, confocal microscopy and flow cytometry demonstrated that the final resulting microspheres are highly uniform both in size (with a diameter of 5 μm) and in fluorescence emission (coefficient of variance < 3%). The Brunauer-Emmett-Teller (BET) surface area of these spheres is 114 m2 g-1. In addition, there are evenly distributed pores with an average size of 20.6 nm on the spheres. These spheres are found to have good thermal stability and photostability, and do not suffer from fluorophore leaching. Fluorescein isothiocyanate (FITC) labelled bovine serum albumin (BSA) as a representative biomolecule can be easily attached onto the fluorescent microspheres. All these characteristics possessed by the APGMA-PDA spheres allow them to be directly used as carriers of biomolecules in lab-on-a-chip immunoassay systems. This journal is

原文English
頁(從 - 到)5249-5255
頁數7
期刊Journal of Materials Chemistry B
2
發行號32
DOIs
出版狀態Published - 2014 八月 28

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)

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