Factors related to contraction and mechanical strength of collagen gels seeded with canine endotenon cells

Meng Yi Chen, Yulong Sun, Chunfeng Zhao, Mark E. Zobitz, Kai Nan An, Steven L. Moran, Peter C. Amadio

研究成果: Article同行評審

10 引文 斯高帕斯(Scopus)

摘要

Fibroblasts can construct a hydrated collagen lattice to a tissue-like structure that is greatly influenced by initial culture conditions. The purpose of this study was to investigate the effects of cell concentration and collagen concentration on the contraction kinetics and mechanical properties of resultant endotenon-derived fibroblast-seeded collagen lattice. The experiment was designed to evaluate the effect of cell concentration (0, 0.25,0.5, and 1.0 × 106 cells/mL) and collagen concentration (0.5, 1.0, 1.5, and 2.0 mg/mL). Collagen lattice contraction was recorded for 42 days, after which time the lattices were mechanically tested. The collagen lattices seeded with higher initial cell concentration had a shorter contraction lag phase (p < 0.01), and exhibited a higher ultimate stress (p < 0.01) and instantaneous and equilibrium modulus (p < 0.01) than those seeded with a lower initial cell concentration. The collagen lattices cultured with a lower initial collagen concentration also had a shorter contraction lag phase, and exhibited greater instantaneous and equilibrium modulus (p < 0.01) than those cultured with higher initial collagen concentration. The collagen lattices of initial 0.5 mg/mL collagen concentration had the highest value of ultimate stress (p < 0.03).

原文English
頁(從 - 到)519-525
頁數7
期刊Journal of Biomedical Materials Research - Part B Applied Biomaterials
82
發行號2
DOIs
出版狀態Published - 2007 七月

All Science Journal Classification (ASJC) codes

  • 生物材料
  • 生物醫學工程

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