Fast and improved bioimaging via temporal focusing multiphoton excitation microscopy with binary digital-micromirror-device holography

Yong Da Sie, Chia Yuan Chang, Chun Yu Lin, Nan Shan Chang, Paul J. Campagnola, Shean Jen Chen

研究成果: Article同行評審

4 引文 斯高帕斯(Scopus)

摘要

Conventional temporal focusing-based multiphoton excitation microscopy (TFMPEM) can offer widefield optical sectioning with an axial excitation confinement of a few microns. To improve the axial confinement of TFMPEM, a binary computer-generated Fourier hologram (CGFH) via a digital-micromirror-device (DMD) was implemented to intrinsically improve the axial confinement by filling the back-focal aperture of the objective lens. Experimental results show that the excitation focal volume can be condensed and the axial confinement improved about 24% according to the DMD holography. In addition, pseudouniform MPE can be achieved using two complementary CGFHs with rapid pulse-width modulation switching via the DMD. Furthermore, bioimaging of CV-1 in origin with SV40 genes-7 cells demonstrates that the TFMPEM with binary DMD holography can improve image quality by enhancing axial excitation confinement and rejecting out-of-focus excitation.

原文English
文章編號116502
期刊Journal of Biomedical Optics
23
發行號11
DOIs
出版狀態Published - 2018

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 生物材料
  • 原子與分子物理與光學
  • 生物醫學工程

指紋

深入研究「Fast and improved bioimaging via temporal focusing multiphoton excitation microscopy with binary digital-micromirror-device holography」主題。共同形成了獨特的指紋。

引用此