Fast DNA hybridization chip by controling temperature and velocity of nucleic acids

Yung Chiang Chung, Yu-Cheng Lin, Wei Chieh Liao, Po Wen Chen, Fong Jian Sie, Chia Ming Hu

研究成果: Conference contribution

摘要

We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in fluorescence intensity was apparent when the temperature was higher than 82°C, and the fluorescence intensity reached an asymptotic value as T > 90°C. A mathematical model was proposed to relate the fluorescence intensity of DNA hybridization with the temperature of hot region and the velocity of cold region. Based on these results, the new hybridization chip with the processes of temperature and velocity differences will provide additional efficiency in DNA detection.

原文English
主出版物標題2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
頁面108-111
頁數4
DOIs
出版狀態Published - 2009
事件2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 - Tainan, Taiwan
持續時間: 2009 十月 182009 十月 21

Other

Other2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
國家/地區Taiwan
城市Tainan
期間09-10-1809-10-21

All Science Journal Classification (ASJC) codes

  • 分子醫學
  • 藥理

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