Fast DNA hybridization chip using denature and motion of nucleic acids

Yung Chiang Chung, Yu-Cheng Lin, Chaung Di Chueh, Chuan You Ye, Li Wei Lai, Wei Chieh Liao

研究成果: Conference contribution

摘要

We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in fluorescence intensity was apparent when the temperature was higher than 82°C, and the fluorescence intensity reached an asymptotic value as T > 90°C. A mathematical model was proposed to relate the fluorescence intensity of DNA hybridization with the temperature of hot region and the velocity of cold region. Based on these results, the new hybridization chip with the processes of temperature and velocity differences will provide additional efficiency in DNA detection.

原文English
主出版物標題International Microsystems Packaging Assembly and Circuits Technology Conference, IMPACT 2010 and International 3D IC Conference, Proceedings
DOIs
出版狀態Published - 2010
事件2010 5th International Microsystems, Packaging, Assembly and Circuits Technology Conference, IMPACT 2010 and International 3D IC Conference - Taipei, Taiwan
持續時間: 2010 10月 202010 10月 22

Other

Other2010 5th International Microsystems, Packaging, Assembly and Circuits Technology Conference, IMPACT 2010 and International 3D IC Conference
國家/地區Taiwan
城市Taipei
期間10-10-2010-10-22

All Science Journal Classification (ASJC) codes

  • 硬體和架構
  • 電氣與電子工程

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